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10.1074/mcp.RA120.002314 http://scihub22266oqcxt.onion/10.1074/mcp.RA120.002314 33109704!8027275!33109704     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell+Proteomics 2021 ; 20 (ä): 100059 Nephropedia Template TP {{tp|p=33109704|t=2021. Antibody Binding Epitope Mapping (AbMap) of Hundred Antibodies in a Single Run |pdf=|usr=}}{{33109704}} gab.com Text Antibody Binding Epitope Mapping (AbMap) of Hundred Antibodies in a Single Run JO: Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=33109704 #FOAvip Antibodies play essential roles in both diagnostics and therapeutics. Epitope mapping is essential to understand how an antibody works and to protect intellectual property. Given the millions of antibodies for which epitope information is lacking, there is a need for high-throughput epitope mapping. To address this, we developed a strategy, Antibody binding epitope Mapping (AbMap), by combining a phage displayed peptide library with next-generation sequencing. Using AbMap, profiles of the peptides bound by 202 antibodies were determined in a single test, and linear epitopes were identified for >50% of the antibodies. Using spike protein (S1 and S2)-enriched antibodies from the convalescent serum of one COVID-19 patient as the input, both linear and potentially conformational epitopes of spike protein specific antibodies were identified. We defined peptide-binding profile of an antibody as the binding capacity (BiC). Conceptually, the BiC could serve as a systematic and functional descriptor of any antibody. Requiring at least one order of magnitude less time and money to map linear epitopes than traditional technologies, AbMap allows for high-throughput epitope mapping and creates many possibilities. Twit Text FOAVip Antibody Binding Epitope Mapping (AbMap) of Hundred Antibodies in a Single Run ????Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=33109704 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33109704 #FOAvip English Wikipedia <ref>{{Cite pmid|33109704}}</ref> Antibody Binding Epitope Mapping (AbMap) of Hundred Antibodies in a Single Run #MMPMID33109704 Qi H; Ma M; Hu C; Xu ZW; Wu FL; Wang N; Lai DY; Li Y; Zhang H; Jiang HW; Meng QF; Guo S; Kang Y; Zhao X; Li H; Tao SC Mol Cell Proteomics 2021[]; 20 (ä): 100059 PMID33109704show ga Antibodies play essential roles in both diagnostics and therapeutics. Epitope mapping is essential to understand how an antibody works and to protect intellectual property. Given the millions of antibodies for which epitope information is lacking, there is a need for high-throughput epitope mapping. To address this, we developed a strategy, Antibody binding epitope Mapping (AbMap), by combining a phage displayed peptide library with next-generation sequencing. Using AbMap, profiles of the peptides bound by 202 antibodies were determined in a single test, and linear epitopes were identified for >50% of the antibodies. Using spike protein (S1 and S2)-enriched antibodies from the convalescent serum of one COVID-19 patient as the input, both linear and potentially conformational epitopes of spike protein specific antibodies were identified. We defined peptide-binding profile of an antibody as the binding capacity (BiC). Conceptually, the BiC could serve as a systematic and functional descriptor of any antibody. Requiring at least one order of magnitude less time and money to map linear epitopes than traditional technologies, AbMap allows for high-throughput epitope mapping and creates many possibilities. |Antibodies, Monoclonal/immunology/metabolism[MESH] |Antibodies, Viral/metabolism[MESH] |COVID-19/*immunology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Epitope Mapping/*methods[MESH] |Epitopes/metabolism[MESH] |Escherichia coli Proteins/immunology[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |Immune Sera/blood/immunology[MESH] |Peptide Library[MESH]Antibody Binding Epitope Mapping (AbMap) of Hundred Antibodies in a Si(epmc).pdf DeepDyve Pubget Overpricing