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10.1016/j.coisb.2020.10.008

http://scihub22266oqcxt.onion/10.1016/j.coisb.2020.10.008
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33102951!7568503!33102951
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suck abstract from ncbi


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pmid33102951      Curr+Opin+Syst+Biol 2020 ; 24 (ä): 93-99
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  • Beyond bulk single-chain sequencing: Getting at the whole receptor #MMPMID33102951
  • Curtis NC; Lee J
  • Curr Opin Syst Biol 2020[Dec]; 24 (ä): 93-99 PMID33102951show ga
  • Recent advancements in paired B-cell receptor sequencing technologies have accelerated the development of simpler, high-throughput pipelines for generating native antibody heavy and light chain pairs used to elucidate novel antibodies and provide insights into antibody response against pathogenic targets. These technologies involve single-cell isolation, using either single wells or emulsified droplets to maintain physical separation of individual cells, followed by sequencing. The development of novel single wells and emulsion-based workflows addresses key challenges by improving throughput of single-cell analyses, reducing method complexity, and integrating functional assays into existing workflows. Enabled by paired B-cell receptor sequencing, functional characterization of pathogen-specific antibodies reveals immunological insights beyond bulk sequencing.
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