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10.4269/ajtmh.20-1079

http://scihub22266oqcxt.onion/10.4269/ajtmh.20-1079
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33098286!7695063!33098286
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suck abstract from ncbi


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pmid33098286      Am+J+Trop+Med+Hyg 2020 ; 103 (6): 2350-2352
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  • A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2 #MMPMID33098286
  • Lau YL; Ismail IB; Izati Binti Mustapa N; Lai MY; Tuan Soh TS; Hassan AH; Peariasamy KM; Lee YL; Goh PP
  • Am J Trop Med Hyg 2020[Dec]; 103 (6): 2350-2352 PMID33098286show ga
  • A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
  • |*Pandemics[MESH]
  • |COVID-19 Testing/methods/*standards[MESH]
  • |COVID-19/*diagnosis/*epidemiology/mortality/transmission[MESH]
  • |Humans[MESH]
  • |Molecular Diagnostic Techniques/methods/*standards[MESH]
  • |Nucleic Acid Amplification Techniques/methods/*standards[MESH]
  • |RNA, Viral/*genetics[MESH]
  • |Reverse Transcription[MESH]
  • |SARS-CoV-2/*genetics/pathogenicity[MESH]


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