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10.1136/thoraxjnl-2020-215705

http://scihub22266oqcxt.onion/10.1136/thoraxjnl-2020-215705
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33097604!ä!33097604

suck abstract from ncbi


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pmid33097604      Thorax 2021 ; 76 (1): 86-88
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  • Use of exhaled breath condensate (EBC) in the diagnosis of SARS-COV-2 (COVID-19) #MMPMID33097604
  • Ryan DJ; Toomey S; Madden SF; Casey M; Breathnach OS; Morris PG; Grogan L; Branagan P; Costello RW; De Barra E; Hurley K; Gunaratnam C; McElvaney NG; OBrien ME; Sulaiman I; Morgan RK; Hennessy BT
  • Thorax 2021[Jan]; 76 (1): 86-88 PMID33097604show ga
  • False negatives from nasopharyngeal swabs (NPS) using reverse transcriptase PCR (RT-PCR) in SARS-CoV-2 are high. Exhaled breath condensate (EBC) contains lower respiratory droplets that may improve detection. We performed EBC RT-PCR for SARS-CoV-2 genes (E, S, N, ORF1ab) on NPS-positive (n=16) and NPS-negative/clinically positive COVID-19 patients (n=15) using two commercial assays. EBC detected SARS-CoV-2 in 93.5% (29/31) using the four genes. Pre-SARS-CoV-2 era controls (n=14) were negative. EBC was positive in NPS negative/clinically positive patients in 66.6% (10/15) using the identical E and S (E/S) gene assay used for NPS, 73.3% (11/15) using the N/ORF1ab assay and 14/15 (93.3%) combined.
  • |*Exhalation[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Breath Tests/*methods[MESH]
  • |COVID-19 Testing/*methods[MESH]
  • |COVID-19/*diagnosis/epidemiology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Prospective Studies[MESH]
  • |RNA, Viral/*analysis[MESH]
  • |Reproducibility of Results[MESH]


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