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10.1186/s13287-020-01950-x http://scihub22266oqcxt.onion/10.1186/s13287-020-01950-x 33097094!7582027!33097094     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cell+Res+Ther 2020 ; 11 (1): 448 Nephropedia Template TP {{tp|p=33097094|t=2020. Identification of AAV serotypes for lung gene therapy in human embryonic stem cell-derived lung organoids |pdf=|usr=}}{{33097094}} gab.com Text Identification of AAV serotypes for lung gene therapy in human embryonic stem cell-derived lung organoids JO: Stem Cell Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=33097094 #FOAvip Gene therapy is being investigated for a range of serious lung diseases, such as cystic fibrosis and emphysema. Recombinant adeno-associated virus (rAAV) is a well-established, safe, viral vector for gene delivery with multiple naturally occurring and artificial serotypes available displaying alternate cell, tissue, and species-specific tropisms. Efficient AAV serotypes for the transduction of the conducting airways have been identified for several species; however, efficient serotypes for human lung parenchyma have not yet been identified. Here, we screened the ability of multiple AAV serotypes to transduce lung bud organoids (LBOs)-a model of human lung parenchyma generated from human embryonic stem cells. Microinjection of LBOs allowed us to model transduction from the luminal surface, similar to dosing via vector inhalation. We identified the naturally occurring rAAV2 and rAAV6 serotypes, along with synthetic rAAV6 variants, as having tropism for the human lung parenchyma. Positive staining of LBOs for surfactant proteins B and C confirmed distal lung identity and suggested the suitability of these vectors for the transduction of alveolar type II cells. Our findings establish LBOs as a new model for pulmonary gene therapy and stress the relevance of LBOs as a viral infection model of the lung parenchyma as relevant in SARS-CoV-2 research. Twit Text FOAVip Identification of AAV serotypes for lung gene therapy in human embryonic stem cell-derived lung organoids ????Stem Cell Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=33097094 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33097094 #FOAvip English Wikipedia <ref>{{Cite pmid|33097094}}</ref> Identification of AAV serotypes for lung gene therapy in human embryonic stem cell-derived lung organoids #MMPMID33097094 Meyer-Berg H; Zhou Yang L; Pilar de Lucas M; Zambrano A; Hyde SC; Gill DR Stem Cell Res Ther 2020[Oct]; 11 (1): 448 PMID33097094show ga Gene therapy is being investigated for a range of serious lung diseases, such as cystic fibrosis and emphysema. Recombinant adeno-associated virus (rAAV) is a well-established, safe, viral vector for gene delivery with multiple naturally occurring and artificial serotypes available displaying alternate cell, tissue, and species-specific tropisms. Efficient AAV serotypes for the transduction of the conducting airways have been identified for several species; however, efficient serotypes for human lung parenchyma have not yet been identified. Here, we screened the ability of multiple AAV serotypes to transduce lung bud organoids (LBOs)-a model of human lung parenchyma generated from human embryonic stem cells. Microinjection of LBOs allowed us to model transduction from the luminal surface, similar to dosing via vector inhalation. We identified the naturally occurring rAAV2 and rAAV6 serotypes, along with synthetic rAAV6 variants, as having tropism for the human lung parenchyma. Positive staining of LBOs for surfactant proteins B and C confirmed distal lung identity and suggested the suitability of these vectors for the transduction of alveolar type II cells. Our findings establish LBOs as a new model for pulmonary gene therapy and stress the relevance of LBOs as a viral infection model of the lung parenchyma as relevant in SARS-CoV-2 research. |Cell Line[MESH] |Dependovirus/*genetics/immunology[MESH] |Gene Transfer Techniques[MESH] |Genetic Therapy/*methods[MESH] |Genetic Vectors/genetics[MESH] |Human Embryonic Stem Cells/*cytology[MESH] |Humans[MESH] |Lung Diseases/*therapy[MESH] |Lung/metabolism[MESH] |Models, Biological[MESH] |Organoids/*cytology[MESH]Identification of AAV serotypes for lung gene therapy in human embryon(epmc).pdf DeepDyve Pubget Overpricing