Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33096020&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4(+) T Cells in COVID-19 #MMPMID33096020
Meckiff BJ; Ramirez-Suastegui C; Fajardo V; Chee SJ; Kusnadi A; Simon H; Eschweiler S; Grifoni A; Pelosi E; Weiskopf D; Sette A; Ay F; Seumois G; Ottensmeier CH; Vijayanand P
Cell 2020[Nov]; 183 (5): 1340-1353.e16 PMID33096020show ga
The contribution of CD4(+) T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4(+) T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found increased proportions of cytotoxic follicular helper cells and cytotoxic T helper (T(H)) cells (CD4-CTLs) responding to SARS-CoV-2 and reduced proportion of SARS-CoV-2-reactive regulatory T cells (T(REG)). Importantly, in hospitalized COVID-19 patients, a strong cytotoxic T(FH) response was observed early in the illness, which correlated negatively with antibody levels to SARS-CoV-2 spike protein. Polyfunctional T(H)1 and T(H)17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4(+) T cells compared to influenza-reactive CD4(+) T cells. Together, our analyses provide insights into the gene expression patterns of SARS-CoV-2-reactive CD4(+) T cells in distinct disease severities.
free
free
free
Cell 2020 ; 183 (5): 1340-1353.e16
