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10.3390/cells9102322 http://scihub22266oqcxt.onion/10.3390/cells9102322 33086697!7589090!33086697     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33086697&cmd=llinks): Failed to open stream: HTTP request failed! 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Effects of Coronavirus Persistence on the Genome Structure and Subsequent Gene Expression, Pathogenicity and Adaptation Capability |pdf=|usr=}}{{33086697}} gab.com Text Effects of Coronavirus Persistence on the Genome Structure and Subsequent Gene Expression, Pathogenicity and Adaptation Capability JO: Cells http://www.kidney.de/pget.php?&tw=1&pmid=33086697 #FOAvip Coronaviruses are able to establish persistence. However, how coronaviruses react to persistence and whether the selected viruses have altered their characteristics remain unclear. In this study, we found that the persistent infection of bovine coronavirus (BCoV), which is in the same genus as SARS-COV-2, led to alterations of genome structure, attenuation of gene expression, and the synthesis of subgenomic mRNA (sgmRNA) with a previously unidentified pattern. Subsequent analyses revealed that the altered genome structures were associated with the attenuation of gene expression. In addition, the genome structure at the 5' terminus and the cellular environment during the persistence were responsible for the sgmRNA synthesis, solving the previously unanswered question regarding the selection of transcription regulatory sequence for synthesis of BCoV sgmRNA 12.7. Although the BCoV variants (BCoV-p95) selected under the persistence replicated efficiently in cells without persistent infection, its pathogenicity was still lower than that of wild-type (wt) BCoV. Furthermore, in comparison with wt BCoV, the variant BCoV-p95 was not able to efficiently adapt to the challenges of alternative environments, suggesting wt BCoV is genetically robust. We anticipate that the findings derived from this fundamental research can contribute to the disease control and treatments against coronavirus infection including SARS-CoV-2. Twit Text FOAVip Effects of Coronavirus Persistence on the Genome Structure and Subsequent Gene Expression, Pathogenicity and Adaptation Capability ????Cells http://www.kidney.de/pget.php?&tw=1&pmid=33086697 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33086697 #FOAvip English Wikipedia <ref>{{Cite pmid|33086697}}</ref> Effects of Coronavirus Persistence on the Genome Structure and Subsequent Gene Expression, Pathogenicity and Adaptation Capability #MMPMID33086697 Lin CH; Yang CY; Wang M; Ou SC; Lo CY; Tsai TL; Wu HY Cells 2020[Oct]; 9 (10): ä PMID33086697show ga Coronaviruses are able to establish persistence. However, how coronaviruses react to persistence and whether the selected viruses have altered their characteristics remain unclear. In this study, we found that the persistent infection of bovine coronavirus (BCoV), which is in the same genus as SARS-COV-2, led to alterations of genome structure, attenuation of gene expression, and the synthesis of subgenomic mRNA (sgmRNA) with a previously unidentified pattern. Subsequent analyses revealed that the altered genome structures were associated with the attenuation of gene expression. In addition, the genome structure at the 5' terminus and the cellular environment during the persistence were responsible for the sgmRNA synthesis, solving the previously unanswered question regarding the selection of transcription regulatory sequence for synthesis of BCoV sgmRNA 12.7. Although the BCoV variants (BCoV-p95) selected under the persistence replicated efficiently in cells without persistent infection, its pathogenicity was still lower than that of wild-type (wt) BCoV. Furthermore, in comparison with wt BCoV, the variant BCoV-p95 was not able to efficiently adapt to the challenges of alternative environments, suggesting wt BCoV is genetically robust. We anticipate that the findings derived from this fundamental research can contribute to the disease control and treatments against coronavirus infection including SARS-CoV-2. |Animals[MESH] |Betacoronavirus/genetics[MESH] |Cattle[MESH] |Cell Line[MESH] |Computational Biology[MESH] |Coronavirus Infections/pathology/virology[MESH] |Coronavirus, Bovine/*genetics[MESH] |Gene Expression Regulation, Viral/*genetics[MESH] |Genome, Viral/*genetics[MESH] |Humans[MESH] |RNA, Messenger/genetics[MESH] |RNA, Viral/genetics[MESH] |Regulatory Sequences, Ribonucleic Acid/*genetics[MESH] |SARS-CoV-2[MESH]Effects of Coronavirus Persistence on the Genome Structure and Subsequ(epmc).pdf DeepDyve Pubget Overpricing