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10.1080/14789450.2020.1833721 http://scihub22266oqcxt.onion/10.1080/14789450.2020.1833721 33084449!7594187!33084449     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Expert+Rev+Proteomics 2020 ; 17 (9): 633-638 Nephropedia Template TP {{tp|p=33084449|t=2020. Deep mutagenesis in the study of COVID-19: a technical overview for the proteomics community |pdf=|usr=}}{{33084449}} gab.com Text Deep mutagenesis in the study of COVID-19: a technical overview for the proteomics community JO: Expert Rev Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=33084449 #FOAvip INTRODUCTION: The spike (S) of SARS coronavirus 2 (SARS-CoV-2) engages angiotensin-converting enzyme 2 (ACE2) on a host cell to trigger viral-cell membrane fusion and infection. The extracellular region of ACE2 can be administered as a soluble decoy to compete for binding sites on the receptor-binding domain (RBD) of S, but it has only moderate affinity and efficacy. The RBD, which is targeted by neutralizing antibodies, may also change and adapt through mutation as SARS-CoV-2 becomes endemic, posing challenges for therapeutic and vaccine development. AREAS COVERED: Deep mutagenesis is a Big Data approach to characterizing sequence variants. A deep mutational scan of ACE2 expressed on human cells identified mutations that increase S affinity and guided the engineering of a potent and broad soluble receptor decoy. A deep mutational scan of the RBD displayed on the surface of yeast has revealed residues tolerant of mutational changes that may act as a source for drug resistance and antigenic drift. EXPERT OPINION: Deep mutagenesis requires a selection of diverse sequence variants; an in vitro evolution experiment that is tracked with next-generation sequencing. The choice of expression system, diversity of the variant library and selection strategy have important consequences for data quality and interpretation. Twit Text FOAVip Deep mutagenesis in the study of COVID-19: a technical overview for the proteomics community ????Expert Rev Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=33084449 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33084449 #FOAvip English Wikipedia <ref>{{Cite pmid|33084449}}</ref> Deep mutagenesis in the study of COVID-19: a technical overview for the proteomics community #MMPMID33084449 Procko E Expert Rev Proteomics 2020[Sep]; 17 (9): 633-638 PMID33084449show ga INTRODUCTION: The spike (S) of SARS coronavirus 2 (SARS-CoV-2) engages angiotensin-converting enzyme 2 (ACE2) on a host cell to trigger viral-cell membrane fusion and infection. The extracellular region of ACE2 can be administered as a soluble decoy to compete for binding sites on the receptor-binding domain (RBD) of S, but it has only moderate affinity and efficacy. The RBD, which is targeted by neutralizing antibodies, may also change and adapt through mutation as SARS-CoV-2 becomes endemic, posing challenges for therapeutic and vaccine development. AREAS COVERED: Deep mutagenesis is a Big Data approach to characterizing sequence variants. A deep mutational scan of ACE2 expressed on human cells identified mutations that increase S affinity and guided the engineering of a potent and broad soluble receptor decoy. A deep mutational scan of the RBD displayed on the surface of yeast has revealed residues tolerant of mutational changes that may act as a source for drug resistance and antigenic drift. EXPERT OPINION: Deep mutagenesis requires a selection of diverse sequence variants; an in vitro evolution experiment that is tracked with next-generation sequencing. The choice of expression system, diversity of the variant library and selection strategy have important consequences for data quality and interpretation. |Angiotensin-Converting Enzyme 2/*genetics/*metabolism[MESH] |Binding Sites[MESH] |Mutagenesis[MESH] |Mutation[MESH] |Protein Interaction Domains and Motifs[MESH] |SARS-CoV-2/*genetics[MESH]Deep mutagenesis in the study of COVID-19: a technical overview for th(epmc).pdf DeepDyve Pubget Overpricing