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Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Diagn+Microbiol+Infect+Dis 2021 ; 99 (1): 115209 Nephropedia Template TP
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Dacron swab and PBS are acceptable alternatives to flocked swab and viral transport media for SARS-CoV-2 #MMPMID33080426
Kline A; Putnam NE; Youn JH; East A; Das S; Frank KM; Zelazny AM
Diagn Microbiol Infect Dis 2021[Jan]; 99 (1): 115209 PMID33080426show ga
Nasopharyngeal flocked swabs placed in viral transport media (VTM) are the preferred collection methodology for respiratory virus testing. Due to the rapid depletion of available reagents and swabs, we have validated an alternative swab placed in phosphate-buffered saline (PBS) for use in respiratory virus testing in a SARS-CoV-2 real-time polymerase chain reaction assay and a multiplexed respiratory virus panel. We collected nasopharyngeal (NP) swabs and oropharyngeal (OP) swabs from 10 healthy volunteers. Flocked swabs were placed in VTM and alternative swabs in PBS. In this feasibility study, we show that NP collection is better for detection of human material than OP collection, as measured by significantly lower RNase P gene cycle threshold values, and that a Dacron polyester swab in PBS shows equivalent detection of SARS-CoV-2 and RSV to a flocked swab in VTM in contrived specimens. Diluted SARS-CoV-2-positive patient specimens are detectable for up to 72?h at 4? degrees C.