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10.1371/journal.pone.0241029

http://scihub22266oqcxt.onion/10.1371/journal.pone.0241029
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suck abstract from ncbi


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pmid33079951      PLoS+One 2020 ; 15 (10): e0241029
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  • The implementation of a rapid sample preparation method for the detection of SARS-CoV-2 in a diagnostic laboratory in South Africa #MMPMID33079951
  • Marais G; Naidoo M; Hsiao NY; Valley-Omar Z; Smuts H; Hardie D
  • PLoS One 2020[]; 15 (10): e0241029 PMID33079951show ga
  • The SARS-CoV-2 pandemic has resulted in shortages of both critical reagents for nucleic acid purification and highly trained staff as supply chains are strained by high demand, public health measures and frequent quarantining and isolation of staff. This created the need for alternate workflows with limited reliance on specialised reagents, equipment and staff. We present here the validation and implementation of such a workflow for preparing samples for downstream SARS-CoV-2 RT-PCR using liquid handling robots. The rapid sample preparation technique evaluated, which included sample centrifugation and heating prior to RT-PCR, showed a 97.37% (95% CI: 92.55-99.28%) positive percent agreement and 97.30% (95% CI: 90.67-99.52%) negative percent agreement compared to nucleic acid purification-based testing. This method was subsequently adopted as the primary sample preparation method in the Groote Schuur Hospital Virology Diagnostic Laboratory in Cape Town, South Africa.
  • |*Laboratories, Hospital[MESH]
  • |Betacoronavirus/*genetics[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/*diagnosis/*epidemiology/virology[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/*epidemiology/virology[MESH]
  • |RNA, Viral/genetics[MESH]
  • |Reproducibility of Results[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]
  • |Robotics/*methods[MESH]
  • |SARS-CoV-2[MESH]
  • |Sensitivity and Specificity[MESH]
  • |South Africa/epidemiology[MESH]


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