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10.1007/s00011-020-01413-2 http://scihub22266oqcxt.onion/10.1007/s00011-020-01413-2 33079210!7572246!33079210     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Inflamm+Res 2021 ; 70 (1): 7-10 Nephropedia Template TP {{tp|p=33079210|t=2021. Inflammasome formation in the lungs of patients with fatal COVID-19 |pdf=|usr=}}{{33079210}} gab.com Text Inflammasome formation in the lungs of patients with fatal COVID-19 JO: Inflamm Res http://www.kidney.de/pget.php?&tw=1&pmid=33079210 #FOAvip OBJECTIVE: The orf8b protein of the coronavirus SARS-CoV, analogous to SARS-CoV-2, triggers the NLRP3 inflammasome in macrophages in vitro. Deregulated inflammasome-mediated release of interleukin-1 family cytokines is important in hyper-inflammatory syndromes, like happens in SARS-CoV-2-mediated cytokine release syndrome. We propose that an intense inflammasome formation characterizes the lungs of patients with fatal COVID-19 disease due to pneumonia and acute respiratory distress syndrome (ARDS). METHODS: Samples from four patients with confirmed COVID-19 pneumonia who had been hospitalized at the Hospital of the University of Trieste (Italy) and died of ARDS and four lung samples from a historical repository from subjects who had died of cardiopulmonary arrest and had not been placed on mechanical ventilation and without evidence of pulmonary infection at postmortem examination were collected. Pathology samples had been fixed in formalin 10% at time of collection and subsequently embedded in paraffin. We conducted staining for ASC (Apoptosis-associated Speck-like protein containing a Caspase recruitment domain), NLRP3 (NACHT, LRR, and PYD domains-containing protein 3), and cleaved caspase-1. RESULTS: Intense expression of the inflammasome was detected, mainly in leukocytes, within the lungs of all patients with fatal COVID-19 in the areas of lung injury. The number of ASC inflammasome specks per high power fields was significantly higher in the lungs of patients with fatal COVID-19 as compared with the lungs of control subjects (52 +/- 22 vs 6 +/- 3, P = 0.0064). CONCLUSIONS: These findings identify the presence of NLRP3 inflammasome aggregates in the lungs of fatal COVID-19 pneumonia thus providing the potential molecular link between viral infection and cytokine release syndrome. Twit Text FOAVip Inflammasome formation in the lungs of patients with fatal COVID-19 ????Inflamm Res http://www.kidney.de/pget.php?&tw=1&pmid=33079210 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33079210 #FOAvip English Wikipedia <ref>{{Cite pmid|33079210}}</ref> Inflammasome formation in the lungs of patients with fatal COVID-19 #MMPMID33079210 Toldo S; Bussani R; Nuzzi V; Bonaventura A; Mauro AG; Cannata A; Pillappa R; Sinagra G; Nana-Sinkam P; Sime P; Abbate A Inflamm Res 2021[Jan]; 70 (1): 7-10 PMID33079210show ga OBJECTIVE: The orf8b protein of the coronavirus SARS-CoV, analogous to SARS-CoV-2, triggers the NLRP3 inflammasome in macrophages in vitro. Deregulated inflammasome-mediated release of interleukin-1 family cytokines is important in hyper-inflammatory syndromes, like happens in SARS-CoV-2-mediated cytokine release syndrome. We propose that an intense inflammasome formation characterizes the lungs of patients with fatal COVID-19 disease due to pneumonia and acute respiratory distress syndrome (ARDS). METHODS: Samples from four patients with confirmed COVID-19 pneumonia who had been hospitalized at the Hospital of the University of Trieste (Italy) and died of ARDS and four lung samples from a historical repository from subjects who had died of cardiopulmonary arrest and had not been placed on mechanical ventilation and without evidence of pulmonary infection at postmortem examination were collected. Pathology samples had been fixed in formalin 10% at time of collection and subsequently embedded in paraffin. We conducted staining for ASC (Apoptosis-associated Speck-like protein containing a Caspase recruitment domain), NLRP3 (NACHT, LRR, and PYD domains-containing protein 3), and cleaved caspase-1. RESULTS: Intense expression of the inflammasome was detected, mainly in leukocytes, within the lungs of all patients with fatal COVID-19 in the areas of lung injury. The number of ASC inflammasome specks per high power fields was significantly higher in the lungs of patients with fatal COVID-19 as compared with the lungs of control subjects (52 +/- 22 vs 6 +/- 3, P = 0.0064). CONCLUSIONS: These findings identify the presence of NLRP3 inflammasome aggregates in the lungs of fatal COVID-19 pneumonia thus providing the potential molecular link between viral infection and cytokine release syndrome. |*Inflammasomes[MESH] |Adult[MESH] |Aged[MESH] |Autopsy[MESH] |CARD Signaling Adaptor Proteins/analysis/metabolism[MESH] |COVID-19/*pathology[MESH] |Caspase 1/analysis/metabolism[MESH] |Cytokine Release Syndrome/metabolism/pathology[MESH] |Female[MESH] |Heart Arrest/etiology[MESH] |Humans[MESH] |Leukocytes/pathology[MESH] |Lung/*pathology[MESH] |Male[MESH] |Middle Aged[MESH] |NLR Family, Pyrin Domain-Containing 3 Protein/analysis/metabolism[MESH] |Pneumonia, Viral/etiology/pathology[MESH]Inflammasome formation in the lungs of patients with fatal COVID-19 (epmc).pdf DeepDyve Pubget Overpricing