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10.1007/s10096-020-04072-7

http://scihub22266oqcxt.onion/10.1007/s10096-020-04072-7
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33078221!7572153!33078221
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suck abstract from ncbi


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pmid33078221      Eur+J+Clin+Microbiol+Infect+Dis 2021 ; 40 (4): 751-759
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  • Rapid response flow cytometric assay for the detection of antibody responses to SARS-CoV-2 #MMPMID33078221
  • Lapuente D; Maier C; Irrgang P; Hubner J; Peter AS; Hoffmann M; Ensser A; Ziegler K; Winkler TH; Birkholz T; Kremer AE; Steininger P; Korn K; Neipel F; Uberla K; Tenbusch M
  • Eur J Clin Microbiol Infect Dis 2021[Apr]; 40 (4): 751-759 PMID33078221show ga
  • SARS-CoV-2 has emerged as a previously unknown zoonotic coronavirus that spread worldwide causing a serious pandemic. While reliable nucleic acid-based diagnostic assays were rapidly available, only a limited number of validated serological assays were available in the early phase of the pandemic. Here, we evaluated a novel flow cytometric approach to assess spike-specific antibody responses.HEK 293T cells expressing SARS-CoV-2 spike protein in its natural confirmation on the surface were used to detect specific IgG and IgM antibody responses in patient sera by flow cytometry. A soluble angiotensin-converting-enzyme 2 (ACE-2) variant was developed as external standard to quantify spike-specific antibody responses on different assay platforms. Analyses of 201 pre-COVID-19 sera proved a high assay specificity in comparison to commercially available CLIA and ELISA systems, while also revealing the highest sensitivity in specimens from PCR-confirmed SARS-CoV-2-infected patients. The external standard allowed robust quantification of antibody responses among different assay platforms. In conclusion, our newly established flow cytometric assay allows sensitive and quantitative detection of SARS-CoV-2-specific antibodies, which can be easily adopted in different laboratories and does not rely on external supply of assay kits. The flow cytometric assay also provides a blueprint for rapid development of serological tests to other emerging viral infections.
  • |Angiotensin-Converting Enzyme 2[MESH]
  • |Antibodies, Viral/*immunology[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Enzyme-Linked Immunosorbent Assay[MESH]
  • |Flow Cytometry/methods[MESH]
  • |HEK293 Cells[MESH]
  • |Humans[MESH]
  • |Immunoglobulin G/*immunology[MESH]
  • |Immunoglobulin M/*immunology[MESH]
  • |Reference Standards[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/*immunology[MESH]
  • |Sensitivity and Specificity[MESH]


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