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10.1007/s12268-020-1458-3

http://scihub22266oqcxt.onion/10.1007/s12268-020-1458-3
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33078045!7556600!33078045
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suck abstract from ncbi


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pmid33078045      Biospektrum+(Heidelb) 2020 ; 26 (6): 624-627
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  • Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification #MMPMID33078045
  • Behrmann O; Bachmann I; Hufert F; Dame G
  • Biospektrum (Heidelb) 2020[]; 26 (6): 624-627 PMID33078045show ga
  • The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39-42 degrees C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.
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