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10.1074/mcp.RA120.002295

http://scihub22266oqcxt.onion/10.1074/mcp.RA120.002295
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33077685!7876485!33077685
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suck abstract from ncbi


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pmid33077685      Mol+Cell+Proteomics 2021 ; 20 (ä): 100058
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  • Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins #MMPMID33077685
  • Zhang Y; Zhao W; Mao Y; Chen Y; Wang S; Zhong Y; Su T; Gong M; Du D; Lu X; Cheng J; Yang H
  • Mol Cell Proteomics 2021[]; 20 (ä): 100058 PMID33077685show ga
  • The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response. Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides. All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences. The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit. We identified masses that correspond to 157 N-glycans, primarily of the complex type. In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type. Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites' location. Moreover, the N-glycan compositions were conserved among different sizes of subunits. Our study indicates that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous. This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.
  • |Animals[MESH]
  • |Glycosylation[MESH]
  • |Humans[MESH]
  • |Insecta/cytology[MESH]
  • |Polysaccharides/chemistry/*metabolism[MESH]
  • |Recombinant Proteins/genetics/*metabolism[MESH]
  • |Spike Glycoprotein, Coronavirus/genetics/*metabolism[MESH]


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