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10.1016/j.bios.2020.112709 http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112709 33075724!7539830!33075724     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 171 (ä): 112709 Nephropedia Template TP {{tp|p=33075724|t=2021. Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector |pdf=|usr=}}{{33075724}} gab.com Text Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33075724 #FOAvip Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by the World Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diagnosis, and monitoring of COVID-19 patients. Here, we demonstrate rapid label-free electrochemical detection of SARS-CoV-2 antibodies using a commercially available impedance sensing platform. A 16-well plate containing sensing electrodes was pre-coated with receptor binding domain (RBD) of SARS-CoV-2 spike protein, and subsequently tested with samples of anti-SARS-CoV-2 monoclonal antibody CR3022 (0.1 mug/ml, 1.0 mug/ml, 10 mug/ml). Subsequent blinded testing was performed on six serum specimens taken from COVID-19 and non-COVID-19 patients (1:100 dilution factor). The platform was able to differentiate spikes in impedance measurements from a negative control (1% milk solution) for all CR3022 samples. Further, successful differentiation and detection of all positive clinical samples from negative control was achieved. Measured impedance values were consistent when compared to standard ELISA test results showing a strong correlation between them (R(2)=0.9). Detection occurs in less than five minutes and the well-based platform provides a simplified and familiar testing interface that can be readily adaptable for use in clinical settings. Twit Text FOAVip Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33075724 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33075724 #FOAvip English Wikipedia <ref>{{Cite pmid|33075724}}</ref> Rapid detection of SARS-CoV-2 antibodies using electrochemical impedance-based detector #MMPMID33075724 Rashed MZ; Kopechek JA; Priddy MC; Hamorsky KT; Palmer KE; Mittal N; Valdez J; Flynn J; Williams SJ Biosens Bioelectron 2021[Jan]; 171 (ä): 112709 PMID33075724show ga Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was classified as a pandemic by the World Health Organization and has caused over 550,000 deaths worldwide as of July 2020. Accurate and scalable point-of-care devices would increase screening, diagnosis, and monitoring of COVID-19 patients. Here, we demonstrate rapid label-free electrochemical detection of SARS-CoV-2 antibodies using a commercially available impedance sensing platform. A 16-well plate containing sensing electrodes was pre-coated with receptor binding domain (RBD) of SARS-CoV-2 spike protein, and subsequently tested with samples of anti-SARS-CoV-2 monoclonal antibody CR3022 (0.1 mug/ml, 1.0 mug/ml, 10 mug/ml). Subsequent blinded testing was performed on six serum specimens taken from COVID-19 and non-COVID-19 patients (1:100 dilution factor). The platform was able to differentiate spikes in impedance measurements from a negative control (1% milk solution) for all CR3022 samples. Further, successful differentiation and detection of all positive clinical samples from negative control was achieved. Measured impedance values were consistent when compared to standard ELISA test results showing a strong correlation between them (R(2)=0.9). Detection occurs in less than five minutes and the well-based platform provides a simplified and familiar testing interface that can be readily adaptable for use in clinical settings. |*Clinical Laboratory Techniques/economics[MESH] |Antibodies, Viral/*blood/immunology[MESH] |Betacoronavirus/*immunology[MESH] |Biosensing Techniques/economics/*instrumentation[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Coronavirus Infections/*blood/diagnosis/economics/immunology[MESH] |Dielectric Spectroscopy/economics/*instrumentation[MESH] |Electric Impedance[MESH] |Equipment Design[MESH] |Humans[MESH] |Immobilized Proteins/immunology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*blood/diagnosis/immunology[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Spike Glycoprotein, Coronavirus/immunology[MESH]Rapid detection of SARS-CoV-2 antibodies using electrochemical impedan(epmc).pdf DeepDyve Pubget Overpricing