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10.1038/s41598-020-74656-y http://scihub22266oqcxt.onion/10.1038/s41598-020-74656-y 33060796!7566627!33060796     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2020 ; 10 (1): 17492 Nephropedia Template TP {{tp|p=33060796|t=2020. Rapid genomic characterization of SARS-CoV-2 viruses from clinical specimens using nanopore sequencing |pdf=|usr=}}{{33060796}} gab.com Text Rapid genomic characterization of SARS-CoV-2 viruses from clinical specimens using nanopore sequencing JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33060796 #FOAvip The novel SARS-CoV-2 outbreak has swiftly spread worldwide. The rapid genome sequencing of SARS-CoV-2 strains has become a helpful tool for better understanding the genomic characteristics and origin of the virus. To obtain virus whole-genome sequences directly from clinical specimens, we performed nanopore sequencing using a modified ARTIC protocol in a portable nanopore sequencer and validated a routine 8-h workflow and a 5-h rapid pipeline. We conducted some optimization to improve the genome sequencing workflow. The sensitivity of the workflow was also tested by serially diluting RNA from clinical samples. The optimized pipeline was finally applied to obtain the whole genomes of 29 clinical specimens collected in Hangzhou from January to March 2020. In the 29 obtained complete genomes of SARS-CoV-2, 33 variations were identified and analyzed. The genomic variations and phylogenetic analysis hinted at multiple sources and different transmission patterns during the COVID-19 epidemic in Hangzhou, China. In conclusion, the genomic characteristics and origin of the virus can be quickly determined by nanopore sequencing following our workflows. Twit Text FOAVip Rapid genomic characterization of SARS-CoV-2 viruses from clinical specimens using nanopore sequencing ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33060796 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33060796 #FOAvip English Wikipedia <ref>{{Cite pmid|33060796}}</ref> Rapid genomic characterization of SARS-CoV-2 viruses from clinical specimens using nanopore sequencing #MMPMID33060796 Li J; Wang H; Mao L; Yu H; Yu X; Sun Z; Qian X; Cheng S; Chen S; Chen J; Pan J; Shi J; Wang X Sci Rep 2020[Oct]; 10 (1): 17492 PMID33060796show ga The novel SARS-CoV-2 outbreak has swiftly spread worldwide. The rapid genome sequencing of SARS-CoV-2 strains has become a helpful tool for better understanding the genomic characteristics and origin of the virus. To obtain virus whole-genome sequences directly from clinical specimens, we performed nanopore sequencing using a modified ARTIC protocol in a portable nanopore sequencer and validated a routine 8-h workflow and a 5-h rapid pipeline. We conducted some optimization to improve the genome sequencing workflow. The sensitivity of the workflow was also tested by serially diluting RNA from clinical samples. The optimized pipeline was finally applied to obtain the whole genomes of 29 clinical specimens collected in Hangzhou from January to March 2020. In the 29 obtained complete genomes of SARS-CoV-2, 33 variations were identified and analyzed. The genomic variations and phylogenetic analysis hinted at multiple sources and different transmission patterns during the COVID-19 epidemic in Hangzhou, China. In conclusion, the genomic characteristics and origin of the virus can be quickly determined by nanopore sequencing following our workflows. |*Genome, Viral[MESH] |Adolescent[MESH] |Adult[MESH] |Betacoronavirus/classification/*genetics/isolation & purification[MESH] |COVID-19[MESH] |Child[MESH] |Coronavirus Infections/diagnosis/virology[MESH] |Female[MESH] |Genetic Variation[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Mutation, Missense[MESH] |Nanopore Sequencing/*methods[MESH] |Pandemics[MESH] |Phylogeny[MESH] |Pneumonia, Viral/diagnosis/virology[MESH] |SARS-CoV-2[MESH] |Sequence Analysis, DNA[MESH]Rapid genomic characterization of SARS-CoV-2 viruses from clinical spe(epmc).pdf DeepDyve Pubget Overpricing