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10.1016/j.cca.2020.10.008 http://scihub22266oqcxt.onion/10.1016/j.cca.2020.10.008 33058839!7550050!33058839     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Chim+Acta 2020 ; 511 (ä): 143-148 Nephropedia Template TP {{tp|p=33058839|t=2020. Evaluation of the clinical performance of single-, dual-, and triple-target SARS-CoV-2 RT-qPCR methods |pdf=|usr=}}{{33058839}} gab.com Text Evaluation of the clinical performance of single-, dual-, and triple-target SARS-CoV-2 RT-qPCR methods JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33058839 #FOAvip BACKGROUND: The coronavirus disease 2019 (COVID-19) has become a pandemic. Reverse transcription quantitative PCR (RT-qPCR) has played a vital role in the diagnosis of COVID-19, but the rates of false negatives is not ideal in dealing with this highly infectious virus. It is thus necessary to systematically evaluate the clinical performance of the single-, dual-, triple-target detection kits to guide the clinical diagnosis of this disease. METHODS: A series of reference materials calibrated by droplet digital PCR (ddPCR) and 57 clinical samples were used to evaluate the clinical performance of six single-, dual-, triple-target SARS-CoV-2 nucleic acid detection kits based on RT-qPCR. RESULTS: The dual-target kits, kit B and kit C had the highest and the lowest detection sensitivity, which was 125 copies/mL and 4000 copies/mL, respectively. Among the 57 clinical samples from patients with COVID-19, 47 were tested positive by the kit B, while 35, 29, 28, 30, and 29 were found positive by the kits A, C, D, E, and F, respectively. The number of targets in a detection kit is not a key factor affecting sensitivity, while the amount of sample loading may influence the performance of a detection kit. CONCLUSIONS: This study provides a guide when choosing or developing a nucleic acid detection kit for the diagnosis of COVID-19. Also, the absolute-quantification feature and high-sensitivity performance of ddPCR, suggesting that it can be used to review clinically suspected samples. Twit Text FOAVip Evaluation of the clinical performance of single-, dual-, and triple-target SARS-CoV-2 RT-qPCR methods ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33058839 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33058839 #FOAvip English Wikipedia <ref>{{Cite pmid|33058839}}</ref> Evaluation of the clinical performance of single-, dual-, and triple-target SARS-CoV-2 RT-qPCR methods #MMPMID33058839 Hu X; Zhu L; Luo Y; Zhao Q; Tan C; Chen X; Zhang H; Hu X; Lu L; Xiao Y; Huang S; He Y; Sim JXL; Su S; Wang F; Peng Y; Wang J; Guo Y; Zhong T Clin Chim Acta 2020[Dec]; 511 (ä): 143-148 PMID33058839show ga BACKGROUND: The coronavirus disease 2019 (COVID-19) has become a pandemic. Reverse transcription quantitative PCR (RT-qPCR) has played a vital role in the diagnosis of COVID-19, but the rates of false negatives is not ideal in dealing with this highly infectious virus. It is thus necessary to systematically evaluate the clinical performance of the single-, dual-, triple-target detection kits to guide the clinical diagnosis of this disease. METHODS: A series of reference materials calibrated by droplet digital PCR (ddPCR) and 57 clinical samples were used to evaluate the clinical performance of six single-, dual-, triple-target SARS-CoV-2 nucleic acid detection kits based on RT-qPCR. RESULTS: The dual-target kits, kit B and kit C had the highest and the lowest detection sensitivity, which was 125 copies/mL and 4000 copies/mL, respectively. Among the 57 clinical samples from patients with COVID-19, 47 were tested positive by the kit B, while 35, 29, 28, 30, and 29 were found positive by the kits A, C, D, E, and F, respectively. The number of targets in a detection kit is not a key factor affecting sensitivity, while the amount of sample loading may influence the performance of a detection kit. CONCLUSIONS: This study provides a guide when choosing or developing a nucleic acid detection kit for the diagnosis of COVID-19. Also, the absolute-quantification feature and high-sensitivity performance of ddPCR, suggesting that it can be used to review clinically suspected samples. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |COVID-19/*diagnosis/*genetics[MESH] |Cohort Studies[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods/*standards[MESH] |Reverse Transcription/genetics[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH]Evaluation of the clinical performance of single-, dual-, and triple-t(epmc).pdf DeepDyve Pubget Overpricing