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10.1038/s41598-020-74380-7 http://scihub22266oqcxt.onion/10.1038/s41598-020-74380-7 33057113!7560892!33057113     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2020 ; 10 (1): 17258 Nephropedia Template TP {{tp|p=33057113|t=2020. Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue |pdf=|usr=}}{{33057113}} gab.com Text Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33057113 #FOAvip Quantitative real time polymerase chain reaction (qPCR) data are normalised using endogenous control genes. We aimed to: (1) demonstrate a pathway to identify endogenous control genes for qPCR analysis of formalin-fixed paraffin-embedded (FFPE) tissue using bladder cancer as an exemplar; and (2) examine the influence of probe length and sample age on PCR amplification and co-expression of candidate genes on apparent expression stability. RNA was extracted from prospective and retrospective samples and subject to qPCR using TaqMan human endogenous control arrays or single tube assays. Gene stability ranking was assessed using coefficient of variation (CoV), GeNorm and NormFinder. Co-expressed genes were identified from The Cancer Genome Atlas (TCGA) using the on-line gene regression analysis tool GRACE. Cycle threshold (Ct) values were lower for prospective (19.49 +/- 2.53) vs retrospective (23.8 +/- 3.32) tissues (p < 0.001) and shorter vs longer probes. Co-expressed genes ranked as the most stable genes in the TCGA cohort by GeNorm when analysed together but ranked lower when analysed individually omitting co-expressed genes indicating bias. Stability values were < 1.5 for the 20 candidate genes in the prospective cohort. As they consistently ranked in the top ten by CoV, GeNorm and Normfinder, UBC, RPLP0, HMBS, GUSB, and TBP are the most suitable endogenous control genes for bladder cancer qPCR. Twit Text FOAVip Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33057113 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33057113 #FOAvip English Wikipedia <ref>{{Cite pmid|33057113}}</ref> Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue #MMPMID33057113 Smith TAD; AbdelKarem OA; Irlam-Jones JJ; Lane B; Valentine H; Bibby BAS; Denley H; Choudhury A; West CML Sci Rep 2020[Oct]; 10 (1): 17258 PMID33057113show ga Quantitative real time polymerase chain reaction (qPCR) data are normalised using endogenous control genes. We aimed to: (1) demonstrate a pathway to identify endogenous control genes for qPCR analysis of formalin-fixed paraffin-embedded (FFPE) tissue using bladder cancer as an exemplar; and (2) examine the influence of probe length and sample age on PCR amplification and co-expression of candidate genes on apparent expression stability. RNA was extracted from prospective and retrospective samples and subject to qPCR using TaqMan human endogenous control arrays or single tube assays. Gene stability ranking was assessed using coefficient of variation (CoV), GeNorm and NormFinder. Co-expressed genes were identified from The Cancer Genome Atlas (TCGA) using the on-line gene regression analysis tool GRACE. Cycle threshold (Ct) values were lower for prospective (19.49 +/- 2.53) vs retrospective (23.8 +/- 3.32) tissues (p < 0.001) and shorter vs longer probes. Co-expressed genes ranked as the most stable genes in the TCGA cohort by GeNorm when analysed together but ranked lower when analysed individually omitting co-expressed genes indicating bias. Stability values were < 1.5 for the 20 candidate genes in the prospective cohort. As they consistently ranked in the top ten by CoV, GeNorm and Normfinder, UBC, RPLP0, HMBS, GUSB, and TBP are the most suitable endogenous control genes for bladder cancer qPCR. |*Gene Expression Regulation, Neoplastic[MESH] |Humans[MESH] |Neoplasms/metabolism/*pathology[MESH] |Paraffin Embedding[MESH] |Prospective Studies[MESH] |RNA/metabolism/standards[MESH] |Real-Time Polymerase Chain Reaction/standards[MESH] |Reference Standards[MESH] |Regression Analysis[MESH] |Retrospective Studies[MESH] |Ribosomal Proteins/genetics[MESH]Selection of endogenous control genes for normalising gene expression (epmc).pdf DeepDyve Pubget Overpricing