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10.3390/ijms21207465 http://scihub22266oqcxt.onion/10.3390/ijms21207465 33050422!7590018!33050422     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2020 ; 21 (20): ä Nephropedia Template TP {{tp|p=33050422|t=2020. Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications |pdf=|usr=}}{{33050422}} gab.com Text Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33050422 #FOAvip : The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate-coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization-Ca(2+)-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme-substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases. Twit Text FOAVip Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33050422 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33050422 #FOAvip English Wikipedia <ref>{{Cite pmid|33050422}}</ref> Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications #MMPMID33050422 Krasitskaya VV; Bashmakova EE; Frank LA Int J Mol Sci 2020[Oct]; 21 (20): ä PMID33050422show ga : The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate-coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization-Ca(2+)-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme-substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases. |*Research[MESH] |Calcium/metabolism[MESH] |Enzyme Activation[MESH] |Firefly Luciferin/metabolism[MESH] |Gene Expression[MESH] |Genes, Reporter[MESH] |Imidazoles/*pharmacology[MESH] |Luciferases/*metabolism[MESH] |Luminescent Measurements/*methods[MESH] |Luminescent Proteins/genetics/metabolism[MESH] |Oxidative Stress[MESH]Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for(epmc).pdf DeepDyve Pubget Overpricing