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10.1002/cpmc.126 http://scihub22266oqcxt.onion/10.1002/cpmc.126 33048448!7646048!33048448     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Protoc+Microbiol 2020 ; 59 (1): e126 Nephropedia Template TP {{tp|p=33048448|t=2020. Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial Chromosome |pdf=|usr=}}{{33048448}} gab.com Text Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial Chromosome JO: Curr Protoc Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33048448 #FOAvip SARS-CoV-2, the causative agent of COVID-19, has been responsible for a million deaths worldwide as of September 2020. At the time of this writing, there are no available US FDA-approved therapeutics for the treatment of SARS-CoV-2 infection. Here, we describe a detailed protocol to generate recombinant (r)SARS-CoV-2 using reverse-genetics approaches based on the use of a bacterial artificial chromosome (BAC). This method will allow the production of mutant rSARS-CoV-2-which is necessary for understanding the function of viral proteins, viral pathogenesis and/or transmission, and interactions at the virus-host interface-and attenuated SARS-CoV-2 to facilitate the discovery of effective countermeasures to control the ongoing SARS-CoV-2 pandemic. (c) 2020 Wiley Periodicals LLC. Basic Protocol: Generation of recombinant SARS-CoV-2 using a bacterial artificial chromosome Support Protocol: Validation and characterization of rSARS-CoV-2. Twit Text FOAVip Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial Chromosome ????Curr Protoc Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33048448 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33048448 #FOAvip English Wikipedia <ref>{{Cite pmid|33048448}}</ref> Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial Chromosome #MMPMID33048448 Chiem K; Ye C; Martinez-Sobrido L Curr Protoc Microbiol 2020[Dec]; 59 (1): e126 PMID33048448show ga SARS-CoV-2, the causative agent of COVID-19, has been responsible for a million deaths worldwide as of September 2020. At the time of this writing, there are no available US FDA-approved therapeutics for the treatment of SARS-CoV-2 infection. Here, we describe a detailed protocol to generate recombinant (r)SARS-CoV-2 using reverse-genetics approaches based on the use of a bacterial artificial chromosome (BAC). This method will allow the production of mutant rSARS-CoV-2-which is necessary for understanding the function of viral proteins, viral pathogenesis and/or transmission, and interactions at the virus-host interface-and attenuated SARS-CoV-2 to facilitate the discovery of effective countermeasures to control the ongoing SARS-CoV-2 pandemic. (c) 2020 Wiley Periodicals LLC. Basic Protocol: Generation of recombinant SARS-CoV-2 using a bacterial artificial chromosome Support Protocol: Validation and characterization of rSARS-CoV-2. |Animals[MESH] |Betacoronavirus/*genetics/growth & development[MESH] |Chlorocebus aethiops[MESH] |Chromosomes, Artificial, Bacterial/*genetics[MESH] |Containment of Biohazards[MESH] |SARS-CoV-2[MESH] |Transfection[MESH]Generation of Recombinant SARS-CoV-2 Using a Bacterial Artificial Chro(epmc).pdf DeepDyve Pubget Overpricing