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10.1016/j.cmi.2020.09.051 http://scihub22266oqcxt.onion/10.1016/j.cmi.2020.09.051 33045370!7547312!33045370     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Microbiol+Infect 2021 ; 27 (2): 286.e7-286.e13 Nephropedia Template TP {{tp|p=33045370|t=2021. A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients |pdf=|usr=}}{{33045370}} gab.com Text A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=33045370 #FOAvip OBJECTIVES: To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. METHODS: We evaluated interferon (IFN)-gamma levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1beta, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-gamma, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-alpha), vascular endothelial growth factor (VEGF) were also evaluated. RESULTS: IFN-gamma-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 'no COVID-19' individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients' clinical parameters. IFN-gamma-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with 'no COVID-19' individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with 'no COVID-19' -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-gamma (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). CONCLUSIONS: Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings. Twit Text FOAVip A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=33045370 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33045370 #FOAvip English Wikipedia <ref>{{Cite pmid|33045370}}</ref> A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients #MMPMID33045370 Petrone L; Petruccioli E; Vanini V; Cuzzi G; Najafi Fard S; Alonzi T; Castilletti C; Palmieri F; Gualano G; Vittozzi P; Nicastri E; Lepore L; Antinori A; Vergori A; Caccamo N; Cantini F; Girardi E; Ippolito G; Grifoni A; Goletti D Clin Microbiol Infect 2021[Feb]; 27 (2): 286.e7-286.e13 PMID33045370show ga OBJECTIVES: To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. METHODS: We evaluated interferon (IFN)-gamma levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1beta, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-gamma, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-alpha), vascular endothelial growth factor (VEGF) were also evaluated. RESULTS: IFN-gamma-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 'no COVID-19' individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients' clinical parameters. IFN-gamma-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with 'no COVID-19' individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with 'no COVID-19' -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-gamma (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). CONCLUSIONS: Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings. |Adult[MESH] |Aged[MESH] |Antigens, Viral/immunology[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/blood/*immunology[MESH] |Cytokines/blood/immunology[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin G/blood/immunology[MESH] |Interferon-gamma/blood/immunology[MESH] |Male[MESH] |Middle Aged[MESH] |SARS-CoV-2/*immunology[MESH] |Spike Glycoprotein, Coronavirus/immunology[MESH] |Th1 Cells/immunology[MESH]A whole blood test to measure SARS-CoV-2-specific response in COVID-19(epmc).pdf DeepDyve Pubget Overpricing