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10.1016/j.ymeth.2020.10.003 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2020.10.003 33045362!7546951!33045362     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods 2022 ; 203 (ä): 594-603 Nephropedia Template TP {{tp|p=33045362|t=2022. CRISPR-based assays for rapid detection of SARS-CoV-2 |pdf=|usr=}}{{33045362}} gab.com Text CRISPR-based assays for rapid detection of SARS-CoV-2 JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=33045362 #FOAvip COVID-19 pandemic posed an unprecedented threat to global public health and economies. There is no effective treatment of the disease, hence, scaling up testing for rapid diagnosis of SARS-CoV-2 infected patients and quarantine them from healthy individuals is one the best strategies to curb the pandemic. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid based tests are considered to be more sensitive with respect to serological tests but present gold standard qRT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. CRISPR based novel techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here, we discuss and present compilation of state-of-the-art detection techniques for COVID-19 using CRISPR technology which has tremendous potential to transform diagnostics and epidemiology. Twit Text FOAVip CRISPR-based assays for rapid detection of SARS-CoV-2 ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=33045362 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33045362 #FOAvip English Wikipedia <ref>{{Cite pmid|33045362}}</ref> CRISPR-based assays for rapid detection of SARS-CoV-2 #MMPMID33045362 Javalkote VS; Kancharla N; Bhadra B; Shukla M; Soni B; Sapre A; Goodin M; Bandyopadhyay A; Dasgupta S Methods 2022[Jul]; 203 (ä): 594-603 PMID33045362show ga COVID-19 pandemic posed an unprecedented threat to global public health and economies. There is no effective treatment of the disease, hence, scaling up testing for rapid diagnosis of SARS-CoV-2 infected patients and quarantine them from healthy individuals is one the best strategies to curb the pandemic. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid based tests are considered to be more sensitive with respect to serological tests but present gold standard qRT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. CRISPR based novel techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here, we discuss and present compilation of state-of-the-art detection techniques for COVID-19 using CRISPR technology which has tremendous potential to transform diagnostics and epidemiology. |*COVID-19/diagnosis[MESH] |*SARS-CoV-2/genetics[MESH] |COVID-19 Testing[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |Pandemics[MESH] |RNA, Viral/genetics[MESH]CRISPR-based assays for rapid detection of SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing