Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.pathol.2020.09.005 http://scihub22266oqcxt.onion/10.1016/j.pathol.2020.09.005 33036772!7508550!33036772     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Pathology 2020 ; 52 (7): 796-800 Nephropedia Template TP {{tp|p=33036772|t=2020. Sample pooling is a viable strategy for SARS-CoV-2 detection in low-prevalence settings |pdf=|usr=}}{{33036772}} gab.com Text Sample pooling is a viable strategy for SARS-CoV-2 detection in low-prevalence settings JO: Pathology http://www.kidney.de/pget.php?&tw=1&pmid=33036772 #FOAvip The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has significantly increased demand on laboratory throughput and reagents for nucleic acid extraction and polymerase chain reaction (PCR). Reagent shortages may limit the expansion of testing required to scale back containment measures. The aims of this study were to investigate the viability of sample pooling as a strategy for increasing test throughput and conserving PCR reagents; and to report our early experience with pooling of clinical samples. A pre-implementation study was performed to assess the sensitivity and theoretical efficiency of two, four, and eight-sample pools in a real-time reverse transcription PCR-based workflow. A standard operating procedure was developed and implemented in two laboratories during periods of peak demand, inclusive of over 29,000 clinical samples processed in our laboratory. Sensitivity decreased (mean absolute increase in cycle threshold value of 0.6, 2.3, and 3.0 for pools of two, four, and eight samples, respectively) and efficiency increased as pool size increased. Gains from pooling diminished at high disease prevalence. Our standard operating procedure was successfully implemented across two laboratories. Increased workflow complexity imparts a higher risk of errors, and requires risk mitigation strategies. Turnaround time for individual samples increased, hence urgent samples should not be pooled. Pooling is a viable strategy for high-throughput testing of SARS-CoV-2 in low-prevalence settings. Twit Text FOAVip Sample pooling is a viable strategy for SARS-CoV-2 detection in low-prevalence settings ????Pathology http://www.kidney.de/pget.php?&tw=1&pmid=33036772 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33036772 #FOAvip English Wikipedia <ref>{{Cite pmid|33036772}}</ref> Sample pooling is a viable strategy for SARS-CoV-2 detection in low-prevalence settings #MMPMID33036772 Chong BSW; Tran T; Druce J; Ballard SA; Simpson JA; Catton M Pathology 2020[Dec]; 52 (7): 796-800 PMID33036772show ga The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has significantly increased demand on laboratory throughput and reagents for nucleic acid extraction and polymerase chain reaction (PCR). Reagent shortages may limit the expansion of testing required to scale back containment measures. The aims of this study were to investigate the viability of sample pooling as a strategy for increasing test throughput and conserving PCR reagents; and to report our early experience with pooling of clinical samples. A pre-implementation study was performed to assess the sensitivity and theoretical efficiency of two, four, and eight-sample pools in a real-time reverse transcription PCR-based workflow. A standard operating procedure was developed and implemented in two laboratories during periods of peak demand, inclusive of over 29,000 clinical samples processed in our laboratory. Sensitivity decreased (mean absolute increase in cycle threshold value of 0.6, 2.3, and 3.0 for pools of two, four, and eight samples, respectively) and efficiency increased as pool size increased. Gains from pooling diminished at high disease prevalence. Our standard operating procedure was successfully implemented across two laboratories. Increased workflow complexity imparts a higher risk of errors, and requires risk mitigation strategies. Turnaround time for individual samples increased, hence urgent samples should not be pooled. Pooling is a viable strategy for high-throughput testing of SARS-CoV-2 in low-prevalence settings. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/epidemiology[MESH] |Humans[MESH] |Prevalence[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Specimen Handling/*methods[MESH]Sample pooling is a viable strategy for SARS-CoV-2 detection in low-pr(epmc).pdf DeepDyve Pubget Overpricing