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10.1007/s11033-020-05889-3

http://scihub22266oqcxt.onion/10.1007/s11033-020-05889-3
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33025503!7538041!33025503
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suck abstract from ncbi


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pmid33025503      Mol+Biol+Rep 2020 ; 47 (11): 9033-9041
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  • Recent advances in the nucleic acid-based diagnostic tool for coronavirus #MMPMID33025503
  • Prabhakar PK; Lakhanpal J
  • Mol Biol Rep 2020[Nov]; 47 (11): 9033-9041 PMID33025503show ga
  • Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. Nucleic acid based detection tool for SARS-CoV-2.
  • |*Molecular Diagnostic Techniques/methods[MESH]
  • |*Nucleic Acid Amplification Techniques[MESH]
  • |Animals[MESH]
  • |Betacoronavirus/*genetics/isolation & purification[MESH]
  • |COVID-19[MESH]
  • |China[MESH]
  • |Clustered Regularly Interspaced Short Palindromic Repeats[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |Humans[MESH]
  • |Microarray Analysis[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |RNA, Viral/*analysis[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]


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