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10.1038/s41598-020-73616-w http://scihub22266oqcxt.onion/10.1038/s41598-020-73616-w 33024174!7538882!33024174     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2020 ; 10 (1): 16608 Nephropedia Template TP {{tp|p=33024174|t=2020. A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR |pdf=|usr=}}{{33024174}} gab.com Text A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33024174 #FOAvip The technique RT-qPCR for viral RNA detection is the current worldwide strategy used for early detection of the novel coronavirus SARS-CoV-2. RNA extraction is a key pre-analytical step in RT-qPCR, often achieved using commercial kits. However, the magnitude of the COVID-19 pandemic is causing disruptions to the global supply chains used by many diagnostic laboratories to procure the commercial kits required for RNA extraction. Shortage in these essential reagents is even more acute in developing countries with no means to produce kits locally. We sought to find an alternative procedure to replace commercial kits using common reagents found in molecular biology laboratories. Here we report a method for RNA extraction that takes about 40 min to complete ten samples, and is not more laborious than current commercial RNA extraction kits. We demonstrate that this method can be used to process nasopharyngeal swab samples and yields RT-qPCR results comparable to those obtained with commercial kits. Most importantly, this procedure can be easily implemented in any molecular diagnostic laboratory. Frequent testing is crucial for individual patient management as well as for public health decision making in this pandemic. Implementation of this method could maintain crucial testing going despite commercial kit shortages. Twit Text FOAVip A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33024174 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33024174 #FOAvip English Wikipedia <ref>{{Cite pmid|33024174}}</ref> A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR #MMPMID33024174 Wozniak A; Cerda A; Ibarra-Henriquez C; Sebastian V; Armijo G; Lamig L; Miranda C; Lagos M; Solari S; Guzman AM; Quiroga T; Hitschfeld S; Riveras E; Ferres M; Gutierrez RA; Garcia P Sci Rep 2020[Oct]; 10 (1): 16608 PMID33024174show ga The technique RT-qPCR for viral RNA detection is the current worldwide strategy used for early detection of the novel coronavirus SARS-CoV-2. RNA extraction is a key pre-analytical step in RT-qPCR, often achieved using commercial kits. However, the magnitude of the COVID-19 pandemic is causing disruptions to the global supply chains used by many diagnostic laboratories to procure the commercial kits required for RNA extraction. Shortage in these essential reagents is even more acute in developing countries with no means to produce kits locally. We sought to find an alternative procedure to replace commercial kits using common reagents found in molecular biology laboratories. Here we report a method for RNA extraction that takes about 40 min to complete ten samples, and is not more laborious than current commercial RNA extraction kits. We demonstrate that this method can be used to process nasopharyngeal swab samples and yields RT-qPCR results comparable to those obtained with commercial kits. Most importantly, this procedure can be easily implemented in any molecular diagnostic laboratory. Frequent testing is crucial for individual patient management as well as for public health decision making in this pandemic. Implementation of this method could maintain crucial testing going despite commercial kit shortages. |*Real-Time Polymerase Chain Reaction[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Diagnostic Tests, Routine[MESH] |Hot Temperature[MESH] |Humans[MESH] |Hydrogen-Ion Concentration[MESH] |Indicators and Reagents[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |RNA, Viral/*genetics/*isolation & purification[MESH] |Reagent Kits, Diagnostic/supply & distribution[MESH]A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR (epmc).pdf DeepDyve Pubget Overpricing