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Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 PLoS+One 2020 ; 15 (10): e0240076 Nephropedia Template TP
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Correlation of ELISA method with three other automated serological tests for the detection of anti-SARS-CoV-2 antibodies #MMPMID33022019
Nguyen NN; Mutnal MB; Gomez RR; Pham HN; Nguyen LT; Koss W; Rao A; Arroliga AC; Wang L; Wang D; Hua Y; Powell PR; Chen L; McCormack CC; Linz WJ; Mohammad AA
PLoS One 2020[]; 15 (10): e0240076 PMID33022019show ga
Public health emergency of SARS-CoV-2 has facilitated diagnostic testing as a related medical countermeasure against COVID-19 outbreak. Numerous serologic antibody tests have become available through an expedited federal emergency use only process. This paper highlights the analytical characteristic of an ELISA based assay by AnshLabs and three random access immunoassay (RAIA) by DiaSorin, Roche, and Abbott that have been approved for emergency use authorization (EUA), at a tertiary academic center in a low disease-prevalence area. The AnshLabs gave higher estimates of sero-prevalence, over the three RAIA methods. For positive results, AnshLabs had 93.3% and 100% agreement with DiaSorin or Abbott and Roche respectively. For negative results, AnshLabs had 74.3% and 78.3% agreement with DiaSorin and Roche or Abbott respectively. All discrepant samples that were positive by AnshLabs and negative by RAIA tested positive by all-in-one step SARS-CoV-2 Total (COV2T) assay performed on the automated Siemens Advia Centaur XPT analyzer. None of these methods, however, are useful in early diagnosis of SARS-CoV-2.