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10.1016/j.bios.2020.112656

http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112656
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33010706!7518959!33010706
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suck abstract from ncbi


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pmid33010706      Biosens+Bioelectron 2020 ; 170 (ä): 112656
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  • An integrated system of air sampling and simultaneous enrichment for rapid biosensing of airborne coronavirus and influenza virus #MMPMID33010706
  • Kim HR; An S; Hwang J
  • Biosens Bioelectron 2020[Dec]; 170 (ä): 112656 PMID33010706show ga
  • Point-of-care risk assessment (PCRA) for airborne viruses requires a system that can enrich low-concentration airborne viruses dispersed in field environments into a small volume of liquid. In this study, airborne virus particles were collected to a degree above the limit of detection (LOD) for a real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). This study employed an electrostatic air sampler to capture aerosolized test viruses (human coronavirus 229E (HCoV-229E), influenza A virus subtype H1N1 (A/H1N1), and influenza A virus subtype H3N2 (A/H3N2)) in a continuously flowing liquid (aerosol-to-hydrosol (ATH) enrichment) and a concanavalin A (ConA)-coated magnetic particles (CMPs)-installed fluidic channel for simultaneous hydrosol-to-hydrosol (HTH) enrichment. The air sampler's ATH enrichment capacity (EC) was evaluated using the aerosol counting method. In contrast, the HTH EC for the ATH-collected sample was evaluated using transmission-electron-microscopy (TEM)-based image analysis and real-time qRT-PCR assay. For example, the ATH EC for HCoV-229E was up to 67,000, resulting in a viral concentration of 0.08 PFU/mL (in a liquid sample) for a viral epidemic scenario of 1.2 PFU/m(3) (in air). The real-time qRT-PCR assay result for this liquid sample was "non-detectable" however, subsequent HTH enrichment for 10 min caused the "non-detectable" sample to become "detectable" (cycle threshold (CT) value of 33.8 +/- 0.06).
  • |Aerosols/analysis[MESH]
  • |Air Microbiology[MESH]
  • |Biosensing Techniques/economics/*instrumentation[MESH]
  • |Coronavirus 229E, Human/genetics/*isolation & purification[MESH]
  • |Coronavirus Infections/*virology[MESH]
  • |Environmental Monitoring/economics/instrumentation[MESH]
  • |Equipment Design[MESH]
  • |Humans[MESH]
  • |Influenza A Virus, H1N1 Subtype/genetics/*isolation & purification[MESH]
  • |Influenza A Virus, H3N2 Subtype/genetics/*isolation & purification[MESH]
  • |Influenza, Human/*virology[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/instrumentation[MESH]


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