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Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Infect+Dis 2021 ; 223 (1): 10-14 Nephropedia Template TP
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A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting #MMPMID33009908
Hicks SM; Pohl K; Neeman T; McNamara HA; Parsons KM; He JS; Ali SA; Nazir S; Rowntree LC; Nguyen THO; Kedzierska K; Doolan DL; Vinuesa CG; Cook MC; Coatsworth N; Myles PS; Kurth F; Sander LE; Mann GJ; Gruen RL; George AJ; Gardiner EE; Cockburn IA
J Infect Dis 2021[Jan]; 223 (1): 10-14 PMID33009908show ga
Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.