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10.1038/s41596-020-0392-7 http://scihub22266oqcxt.onion/10.1038/s41596-020-0392-7 33005038!7528719!33005038     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2020 ; 15 (11): 3663-3677 Nephropedia Template TP {{tp|p=33005038|t=2020. Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks |pdf=|usr=}}{{33005038}} gab.com Text Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=33005038 #FOAvip The complexity of current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick. Nucleic acids can be purified by dipping in-house-made dipsticks into just three solutions: the extract (to bind the nucleic acids), a wash buffer (to remove impurities) and the amplification reaction (to elute the nucleic acids). The speed and simplicity of this method make it ideally suited for molecular applications, both within and outside the laboratory, including limited-resource settings such as remote field sites and teaching institutions. Detailed instructions for how to easily manufacture large numbers of dipsticks in house are provided. Using the instructions, readers can create more than 200 dipsticks in <30 min and perform dipstick-based nucleic acid purifications in 30 s. Twit Text FOAVip Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=33005038 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33005038 #FOAvip English Wikipedia <ref>{{Cite pmid|33005038}}</ref> Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks #MMPMID33005038 Mason MG; Botella JR Nat Protoc 2020[Nov]; 15 (11): 3663-3677 PMID33005038show ga The complexity of current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick. Nucleic acids can be purified by dipping in-house-made dipsticks into just three solutions: the extract (to bind the nucleic acids), a wash buffer (to remove impurities) and the amplification reaction (to elute the nucleic acids). The speed and simplicity of this method make it ideally suited for molecular applications, both within and outside the laboratory, including limited-resource settings such as remote field sites and teaching institutions. Detailed instructions for how to easily manufacture large numbers of dipsticks in house are provided. Using the instructions, readers can create more than 200 dipsticks in <30 min and perform dipstick-based nucleic acid purifications in 30 s. |Animals[MESH] |Bacteria/chemistry[MESH] |Cellulose/*chemistry[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques/economics/instrumentation/methods[MESH] |Nucleic Acids/genetics/*isolation & purification[MESH] |Plants/chemistry[MESH] |Time Factors[MESH]Rapid (30-second), equipment-free purification of nucleic acids using (epmc).pdf DeepDyve Pubget Overpricing