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10.1038/s41598-020-72812-y http://scihub22266oqcxt.onion/10.1038/s41598-020-72812-y 33004828!7529757!33004828     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2020 ; 10 (1): 16212 Nephropedia Template TP {{tp|p=33004828|t=2020. Time-correlated single molecule localization microscopy enhances resolution and fidelity |pdf=|usr=}}{{33004828}} gab.com Text Time-correlated single molecule localization microscopy enhances resolution and fidelity JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33004828 #FOAvip Single-molecule-localization-microscopy (SMLM) enables superresolution imaging of biological samples down to ~ 10-20 nm and in single molecule detail. However, common SMLM reconstruction largely disregards information embedded in the entire intensity trajectories of individual emitters. Here, we develop and demonstrate an approach, termed time-correlated-SMLM (tcSMLM), that uses such information for enhancing SMLM reconstruction. Specifically, tcSMLM is shown to increase the spatial resolution and fidelity of SMLM reconstruction of both simulated and experimental data; esp. upon acquisition under stringent conditions of low SNR, high acquisition rate and high density of emitters. We further provide detailed guidelines and optimization procedures for effectively applying tcSMLM to data of choice. Importantly, our approach can be readily added in tandem to multiple SMLM and related superresolution reconstruction algorithms. Thus, we expect that our approach will become an effective and readily accessible tool for enhancing SMLM and superresolution imaging. Twit Text FOAVip Time-correlated single molecule localization microscopy enhances resolution and fidelity ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33004828 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33004828 #FOAvip English Wikipedia <ref>{{Cite pmid|33004828}}</ref> Time-correlated single molecule localization microscopy enhances resolution and fidelity #MMPMID33004828 Hermon K; Schidorsky S; Razvag Y; Yakovian O; Sherman E Sci Rep 2020[Oct]; 10 (1): 16212 PMID33004828show ga Single-molecule-localization-microscopy (SMLM) enables superresolution imaging of biological samples down to ~ 10-20 nm and in single molecule detail. However, common SMLM reconstruction largely disregards information embedded in the entire intensity trajectories of individual emitters. Here, we develop and demonstrate an approach, termed time-correlated-SMLM (tcSMLM), that uses such information for enhancing SMLM reconstruction. Specifically, tcSMLM is shown to increase the spatial resolution and fidelity of SMLM reconstruction of both simulated and experimental data; esp. upon acquisition under stringent conditions of low SNR, high acquisition rate and high density of emitters. We further provide detailed guidelines and optimization procedures for effectively applying tcSMLM to data of choice. Importantly, our approach can be readily added in tandem to multiple SMLM and related superresolution reconstruction algorithms. Thus, we expect that our approach will become an effective and readily accessible tool for enhancing SMLM and superresolution imaging. äTime-correlated single molecule localization microscopy enhances resol(epmc).pdf DeepDyve Pubget Overpricing