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10.1016/j.cca.2020.09.033 http://scihub22266oqcxt.onion/10.1016/j.cca.2020.09.033 33002475!7834570!33002475     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Chim+Acta 2020 ; 511 (ä): 28-32 Nephropedia Template TP {{tp|p=33002475|t=2020. SARS-CoV-2 infection serology validation of different methods: Usefulness of IgA in the early phase of infection |pdf=|usr=}}{{33002475}} gab.com Text SARS-CoV-2 infection serology validation of different methods: Usefulness of IgA in the early phase of infection JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33002475 #FOAvip BACKGROUND AND AIMS: A novel coronavirus (SARS-CoV-2) was isolated from the respiratory samples of patients with pneumonia as showed by the sequence analysis of the virus genomes obtained in Wuhan, China. The antibody response to SARS-CoV-2 is not well understood yet, but the availability of sensitive and specific serological assays will be crucial for the early diagnosis of infection, for epidemiological studies and for defining the presence of neutralizing antibodies in response to a possible vaccine. MATERIALS AND METHODS: We tested and compared the performances of one chemiluminescent immunoassay (CLIA), two enzyme-linked immunosorbent assay (ELISA) and an electrochemiluminescence immunoassay (ECLIA). RESULTS: The ECLIA serological assay performed best and may be a valid screening method for SARS-COV-2 infection. The IgA detected by the ELISA assay might be a more reliable and stable early serological marker than IgM. Instead, IgGs, as expected, showed stable level after 10 days from symptoms onset. CONCLUSION: The ECLIA method could be used as screening test, considering both the excellent performance and the cost per single test; while ELISA assay for IgG and IgA, which are present at a higher level than IgM and last longer, might be used as confirmatory test. Twit Text FOAVip SARS-CoV-2 infection serology validation of different methods: Usefulness of IgA in the early phase of infection ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33002475 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33002475 #FOAvip English Wikipedia <ref>{{Cite pmid|33002475}}</ref> SARS-CoV-2 infection serology validation of different methods: Usefulness of IgA in the early phase of infection #MMPMID33002475 Pieri M; Ciotti M; Carlozzi N; Frassanito ML; Meloni A; Cistera A; Turchetti G; Niscola S; Labate G; Calugi G; Bernardini S Clin Chim Acta 2020[Dec]; 511 (ä): 28-32 PMID33002475show ga BACKGROUND AND AIMS: A novel coronavirus (SARS-CoV-2) was isolated from the respiratory samples of patients with pneumonia as showed by the sequence analysis of the virus genomes obtained in Wuhan, China. The antibody response to SARS-CoV-2 is not well understood yet, but the availability of sensitive and specific serological assays will be crucial for the early diagnosis of infection, for epidemiological studies and for defining the presence of neutralizing antibodies in response to a possible vaccine. MATERIALS AND METHODS: We tested and compared the performances of one chemiluminescent immunoassay (CLIA), two enzyme-linked immunosorbent assay (ELISA) and an electrochemiluminescence immunoassay (ECLIA). RESULTS: The ECLIA serological assay performed best and may be a valid screening method for SARS-COV-2 infection. The IgA detected by the ELISA assay might be a more reliable and stable early serological marker than IgM. Instead, IgGs, as expected, showed stable level after 10 days from symptoms onset. CONCLUSION: The ECLIA method could be used as screening test, considering both the excellent performance and the cost per single test; while ELISA assay for IgG and IgA, which are present at a higher level than IgM and last longer, might be used as confirmatory test. |COVID-19 Serological Testing/*methods/*standards[MESH] |COVID-19/*blood/*diagnosis[MESH] |Clinical Laboratory Techniques/methods/standards[MESH] |Enzyme-Linked Immunosorbent Assay/methods/standards[MESH] |Humans[MESH] |Immunoglobulin A/*blood[MESH] |Real-Time Polymerase Chain Reaction/methods/standards[MESH] |Reproducibility of Results[MESH]SARS-CoV-2 infection serology validation of different methods: Usefuln(epmc).pdf DeepDyve Pubget Overpricing