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10.3892/ijmm.2020.4726 http://scihub22266oqcxt.onion/10.3892/ijmm.2020.4726 33000189!7521554!33000189     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Med 2020 ; 46 (5): 1633-1643 Nephropedia Template TP {{tp|p=33000189|t=2020. Alteration of enzymes and their application to nucleic acid amplification (Review) |pdf=|usr=}}{{33000189}} gab.com Text Alteration of enzymes and their application to nucleic acid amplification (Review) JO: Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=33000189 #FOAvip Since the discovery of polymerase chain reaction (PCR) in 1985, several methods have been developed to achieve nucleic acid amplification, and are currently used in various fields including clinical diagnosis and life science research. Thus, a wealth of information has accumulated regarding nucleic acid?related enzymes. In this review, some nucleic acid?related enzymes were selected and the recent advances in their modification along with their application to nucleic acid amplification were described. The discussion also focused on optimization of the corresponding reaction conditions. Using newly developed enzymes under well?optimized reaction conditions, the sensitivity, specificity, and fidelity of nucleic acid tests can be improved successfully. Twit Text FOAVip Alteration of enzymes and their application to nucleic acid amplification (Review) ????Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=33000189 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33000189 #FOAvip English Wikipedia <ref>{{Cite pmid|33000189}}</ref> Alteration of enzymes and their application to nucleic acid amplification (Review) #MMPMID33000189 Yasukawa K; Yanagihara I; Fujiwara S Int J Mol Med 2020[Nov]; 46 (5): 1633-1643 PMID33000189show ga Since the discovery of polymerase chain reaction (PCR) in 1985, several methods have been developed to achieve nucleic acid amplification, and are currently used in various fields including clinical diagnosis and life science research. Thus, a wealth of information has accumulated regarding nucleic acid?related enzymes. In this review, some nucleic acid?related enzymes were selected and the recent advances in their modification along with their application to nucleic acid amplification were described. The discussion also focused on optimization of the corresponding reaction conditions. Using newly developed enzymes under well?optimized reaction conditions, the sensitivity, specificity, and fidelity of nucleic acid tests can be improved successfully. |*Nucleic Acid Amplification Techniques[MESH] |Animals[MESH] |DNA-Binding Proteins/metabolism[MESH] |Enzyme Stability[MESH] |Enzymes/*metabolism[MESH] |Humans[MESH] |Substrate Specificity[MESH]Alteration of enzymes and their application to nucleic acid amplificat(epmc).pdf DeepDyve Pubget Overpricing