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Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Mem+Inst+Oswaldo+Cruz 2020 ; 115 (ä): e200310 Nephropedia Template TP
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Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil #MMPMID32997001
Nascimento VAD; Corado ALG; Nascimento FOD; Costa AKAD; Duarte DCG; Luz SLB; Goncalves LMF; Jesus MS; Costa CFD; Delatorre E; Naveca FG
Mem Inst Oswaldo Cruz 2020[]; 115 (ä): e200310 PMID32997001show ga
A new coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] is currently causing a life-threatening pandemic. In this study, we report the complete genome sequencing and genetic characterisation of a SARS-CoV-2 detected in Manaus, Amazonas, Brazil, and the protocol we designed to generate high-quality SARS-CoV-2 full genome data. The isolate was obtained from an asymptomatic carrier returning from Madrid, Spain. Nucleotide sequence analysis showed a total of nine mutations in comparison with the original human case in Wuhan, China, and support this case as belonging to the recently proposed lineage A.2. Phylogeographic analysis further confirmed the likely European origin of this case. To our knowledge, this is the first SARS-CoV-2 genome obtained from the North Brazilian Region. We believe that the information generated in this study may contribute to the ongoing efforts toward the SARS-CoV-2 emergence.