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10.1126/scisignal.abe1202 http://scihub22266oqcxt.onion/10.1126/scisignal.abe1202 32994211!8028745!32994211     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Signal 2020 ; 13 (651): ä Nephropedia Template TP {{tp|p=32994211|t=2020. High-resolution structures of the SARS-CoV-2 2 -O-methyltransferase reveal strategies for structure-based inhibitor design |pdf=|usr=}}{{32994211}} gab.com Text High-resolution structures of the SARS-CoV-2 2 -O-methyltransferase reveal strategies for structure-based inhibitor design JO: Sci Signal http://www.kidney.de/pget.php?&tw=1&pmid=32994211 #FOAvip There are currently no antiviral therapies specific for SARS-CoV-2, the virus responsible for the global pandemic disease COVID-19. To facilitate structure-based drug design, we conducted an x-ray crystallographic study of the SARS-CoV-2 nsp16-nsp10 2'-O-methyltransferase complex, which methylates Cap-0 viral mRNAs to improve viral protein translation and to avoid host immune detection. We determined the structures for nsp16-nsp10 heterodimers bound to the methyl donor S-adenosylmethionine (SAM), the reaction product S-adenosylhomocysteine (SAH), or the SAH analog sinefungin (SFG). We also solved structures for nsp16-nsp10 in complex with the methylated Cap-0 analog m(7)GpppA and either SAM or SAH. Comparative analyses between these structures and published structures for nsp16 from other betacoronaviruses revealed flexible loops in open and closed conformations at the m(7)GpppA-binding pocket. Bound sulfates in several of the structures suggested the location of the ribonucleic acid backbone phosphates in the ribonucleotide-binding groove. Additional nucleotide-binding sites were found on the face of the protein opposite the active site. These various sites and the conserved dimer interface could be exploited for the development of antiviral inhibitors. Twit Text FOAVip High-resolution structures of the SARS-CoV-2 2 -O-methyltransferase reveal strategies for structure-based inhibitor design ????Sci Signal http://www.kidney.de/pget.php?&tw=1&pmid=32994211 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32994211 #FOAvip English Wikipedia <ref>{{Cite pmid|32994211}}</ref> High-resolution structures of the SARS-CoV-2 2 -O-methyltransferase reveal strategies for structure-based inhibitor design #MMPMID32994211 Rosas-Lemus M; Minasov G; Shuvalova L; Inniss NL; Kiryukhina O; Brunzelle J; Satchell KJF Sci Signal 2020[Sep]; 13 (651): ä PMID32994211show ga There are currently no antiviral therapies specific for SARS-CoV-2, the virus responsible for the global pandemic disease COVID-19. To facilitate structure-based drug design, we conducted an x-ray crystallographic study of the SARS-CoV-2 nsp16-nsp10 2'-O-methyltransferase complex, which methylates Cap-0 viral mRNAs to improve viral protein translation and to avoid host immune detection. We determined the structures for nsp16-nsp10 heterodimers bound to the methyl donor S-adenosylmethionine (SAM), the reaction product S-adenosylhomocysteine (SAH), or the SAH analog sinefungin (SFG). We also solved structures for nsp16-nsp10 in complex with the methylated Cap-0 analog m(7)GpppA and either SAM or SAH. Comparative analyses between these structures and published structures for nsp16 from other betacoronaviruses revealed flexible loops in open and closed conformations at the m(7)GpppA-binding pocket. Bound sulfates in several of the structures suggested the location of the ribonucleic acid backbone phosphates in the ribonucleotide-binding groove. Additional nucleotide-binding sites were found on the face of the protein opposite the active site. These various sites and the conserved dimer interface could be exploited for the development of antiviral inhibitors. |Adenosine/analogs & derivatives/metabolism/pharmacology[MESH] |Betacoronavirus/drug effects/*enzymology[MESH] |Binding Sites[MESH] |COVID-19[MESH] |Catalytic Domain[MESH] |Coronavirus Infections/*drug therapy[MESH] |Crystallography, X-Ray[MESH] |Dimerization[MESH] |Genes, Viral/genetics[MESH] |Humans[MESH] |Methylation[MESH] |Methyltransferases/antagonists & inhibitors/*chemistry[MESH] |Models, Molecular[MESH] |Open Reading Frames/genetics[MESH] |Pandemics[MESH] |Pneumonia, Viral/*drug therapy[MESH] |Protein Binding[MESH] |Protein Conformation[MESH] |RNA Cap Analogs/metabolism[MESH] |RNA Processing, Post-Transcriptional[MESH] |RNA, Viral/metabolism[MESH] |S-Adenosylhomocysteine/metabolism[MESH] |S-Adenosylmethionine/metabolism[MESH] |SARS-CoV-2[MESH] |Structure-Activity Relationship[MESH]High-resolution structures of the SARS-CoV-2 2 -O-methyltransferase re(epmc).pdf DeepDyve Pubget Overpricing