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10.1021/acssensors.0c01742 http://scihub22266oqcxt.onion/10.1021/acssensors.0c01742 32989986!7534800!32989986     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Sens 2020 ; 5 (10): 3043-3048 Nephropedia Template TP {{tp|p=32989986|t=2020. Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs |pdf=|usr=}}{{32989986}} gab.com Text Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs JO: ACS Sens http://www.kidney.de/pget.php?&tw=1&pmid=32989986 #FOAvip Mass testing is fundamental to face the pandemic caused by the coronavirus SARS-CoV-2 discovered at the end of 2019. To this aim, it is necessary to establish reliable, fast, and cheap tools to detect viral particles in biological material so to identify the people capable of spreading the infection. We demonstrate that a colorimetric biosensor based on gold nanoparticle (AuNP) interaction induced by SARS-CoV-2 lends itself as an outstanding tool for detecting viral particles in nasal and throat swabs. The extinction spectrum of a colloidal solution of multiple viral-target gold nanoparticles-AuNPs functionalized with antibodies targeting three surface proteins of SARS-CoV-2 (spike, envelope, and membrane)-is red-shifted in few minutes when mixed with a solution containing the viral particle. The optical density of the mixed solution measured at 560 nm was compared to the threshold cycle (C(t)) of a real-time PCR (gold standard for detecting the presence of viruses) finding that the colorimetric method is able to detect very low viral load with a detection limit approaching that of the real-time PCR. Since the method is sensitive to the infecting viral particle rather than to its RNA, the achievements reported here open a new perspective not only in the context of the current and possible future pandemics, but also in microbiology, as the biosensor proves itself to be a powerful though simple tool for measuring the viral particle concentration. Twit Text FOAVip Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs ????ACS Sens http://www.kidney.de/pget.php?&tw=1&pmid=32989986 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32989986 #FOAvip English Wikipedia <ref>{{Cite pmid|32989986}}</ref> Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs #MMPMID32989986 Ventura BD; Cennamo M; Minopoli A; Campanile R; Censi SB; Terracciano D; Portella G; Velotta R ACS Sens 2020[Oct]; 5 (10): 3043-3048 PMID32989986show ga Mass testing is fundamental to face the pandemic caused by the coronavirus SARS-CoV-2 discovered at the end of 2019. To this aim, it is necessary to establish reliable, fast, and cheap tools to detect viral particles in biological material so to identify the people capable of spreading the infection. We demonstrate that a colorimetric biosensor based on gold nanoparticle (AuNP) interaction induced by SARS-CoV-2 lends itself as an outstanding tool for detecting viral particles in nasal and throat swabs. The extinction spectrum of a colloidal solution of multiple viral-target gold nanoparticles-AuNPs functionalized with antibodies targeting three surface proteins of SARS-CoV-2 (spike, envelope, and membrane)-is red-shifted in few minutes when mixed with a solution containing the viral particle. The optical density of the mixed solution measured at 560 nm was compared to the threshold cycle (C(t)) of a real-time PCR (gold standard for detecting the presence of viruses) finding that the colorimetric method is able to detect very low viral load with a detection limit approaching that of the real-time PCR. Since the method is sensitive to the infecting viral particle rather than to its RNA, the achievements reported here open a new perspective not only in the context of the current and possible future pandemics, but also in microbiology, as the biosensor proves itself to be a powerful though simple tool for measuring the viral particle concentration. |Betacoronavirus/*chemistry[MESH] |Biosensing Techniques[MESH] |COVID-19[MESH] |Colorimetry/*methods[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Gold[MESH] |Humans[MESH] |Membrane Proteins/chemistry[MESH] |Metal Nanoparticles[MESH] |Nasal Mucosa/*virology[MESH] |Pandemics[MESH] |Pharynx/*virology[MESH] |Photochemistry[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH] |Specimen Handling[MESH] |Spike Glycoprotein, Coronavirus/chemistry[MESH] |Threshold Limit Values[MESH]Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat(epmc).pdf DeepDyve Pubget Overpricing