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10.1261/rna.076604.120 http://scihub22266oqcxt.onion/10.1261/rna.076604.120 32989044!7668261!32989044     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32989044&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA 2020 ; 26 (12): 1976-1999 Nephropedia Template TP {{tp|p=32989044|t=2020. Physiologic RNA targets and refined sequence specificity of coronavirus EndoU |pdf=|usr=}}{{32989044}} gab.com Text Physiologic RNA targets and refined sequence specificity of coronavirus EndoU JO: RNA http://www.kidney.de/pget.php?&tw=1&pmid=32989044 #FOAvip Coronavirus EndoU inhibits dsRNA-activated antiviral responses; however, the physiologic RNA substrates of EndoU are unknown. In this study, we used mouse hepatitis virus (MHV)-infected bone marrow-derived macrophage (BMM) and cyclic phosphate cDNA sequencing to identify the RNA targets of EndoU. EndoU targeted viral RNA, cleaving the 3' side of pyrimidines with a strong preference for U ( downward arrow) A and C ( downward arrow) A sequences (endoY ( downward arrow) A). EndoU-dependent cleavage was detected in every region of MHV RNA, from the 5' NTR to the 3' NTR, including transcriptional regulatory sequences (TRS). Cleavage at two CA dinucleotides immediately adjacent to the MHV poly(A) tail suggests a mechanism to suppress negative-strand RNA synthesis and the accumulation of viral dsRNA. MHV with EndoU (EndoU(mut)) or 2'-5' phosphodiesterase (PDE(mut)) mutations provoked the activation of RNase L in BMM, with corresponding cleavage of RNAs by RNase L. The physiologic targets of EndoU are viral RNA templates required for negative-strand RNA synthesis and dsRNA accumulation. Coronavirus EndoU cleaves U ( downward arrow) A and C ( downward arrow) A sequences (endoY ( downward arrow) A) within viral (+) strand RNA to evade dsRNA-activated host responses. Twit Text FOAVip Physiologic RNA targets and refined sequence specificity of coronavirus EndoU ????RNA http://www.kidney.de/pget.php?&tw=1&pmid=32989044 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32989044 #FOAvip English Wikipedia <ref>{{Cite pmid|32989044}}</ref> Physiologic RNA targets and refined sequence specificity of coronavirus EndoU #MMPMID32989044 Ancar R; Li Y; Kindler E; Cooper DA; Ransom M; Thiel V; Weiss SR; Hesselberth JR; Barton DJ RNA 2020[Dec]; 26 (12): 1976-1999 PMID32989044show ga Coronavirus EndoU inhibits dsRNA-activated antiviral responses; however, the physiologic RNA substrates of EndoU are unknown. In this study, we used mouse hepatitis virus (MHV)-infected bone marrow-derived macrophage (BMM) and cyclic phosphate cDNA sequencing to identify the RNA targets of EndoU. EndoU targeted viral RNA, cleaving the 3' side of pyrimidines with a strong preference for U ( downward arrow) A and C ( downward arrow) A sequences (endoY ( downward arrow) A). EndoU-dependent cleavage was detected in every region of MHV RNA, from the 5' NTR to the 3' NTR, including transcriptional regulatory sequences (TRS). Cleavage at two CA dinucleotides immediately adjacent to the MHV poly(A) tail suggests a mechanism to suppress negative-strand RNA synthesis and the accumulation of viral dsRNA. MHV with EndoU (EndoU(mut)) or 2'-5' phosphodiesterase (PDE(mut)) mutations provoked the activation of RNase L in BMM, with corresponding cleavage of RNAs by RNase L. The physiologic targets of EndoU are viral RNA templates required for negative-strand RNA synthesis and dsRNA accumulation. Coronavirus EndoU cleaves U ( downward arrow) A and C ( downward arrow) A sequences (endoY ( downward arrow) A) within viral (+) strand RNA to evade dsRNA-activated host responses. |Animals[MESH] |Cells, Cultured[MESH] |Macrophages/virology[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Murine hepatitis virus/*enzymology[MESH] |Mutation[MESH] |Nucleotide Motifs[MESH] |Protein Binding[MESH] |RNA/*chemistry/metabolism[MESH] |Uridylate-Specific Endoribonucleases/genetics/*metabolism[MESH]Physiologic RNA targets and refined sequence specificity of coronaviru(epmc).pdf DeepDyve Pubget Overpricing