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10.3389/fmed.2020.00504

http://scihub22266oqcxt.onion/10.3389/fmed.2020.00504
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32984381!7479817!32984381
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suck abstract from ncbi


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pmid32984381      Front+Med+(Lausanne) 2020 ; 7 (ä): 504
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  • Germ-Free Mice Under Two-Layer Textiles Are Fully Protected From Bacteria in Sprayed Microdroplets: A Functional in vivo Test Method of Facemask/Filtration Materials #MMPMID32984381
  • Rodriguez-Palacios A; Conger M; Cominelli F
  • Front Med (Lausanne) 2020[]; 7 (ä): 504 PMID32984381show ga
  • Several studies have measured the effectiveness of masks at retaining particles of various sizes in vitro. To identify a functional in vivo model, herein we used germ-free (GF) mice to test the effectiveness of textiles as filtration material and droplet barriers to complement available in vitro-based knowledge. Herein, we report a study conducted in vivo with bacteria-carrying microdroplets to determine to what extent household textiles prevent contamination of GF mice in their environment. Using a recently validated spray-simulation method (mimicking a sneeze), herein we first determined that combed-cotton textiles used as two-layer-barriers covering the mouse cages prevented the contamination of all GF animals when sprayed 10-20 bacterial-droplet units/cm(2). In additional to exposure trials, the model showed that GF mice were again protected by the combed-cotton textile after the acute exposure to 10 times more droplets (20 "spray-sneezes", ~200 bacterial-droplet units/cm(2)). Overall, two-layer combed-cotton protected 100% of the GF mice from bacteria-carrying droplets (n = 20 exposure-events), which was significantly superior compared to 100% mouse contamination without textile coverage or when 95% partly covered (n = 18, Fisher-exact, p < 0.0001). Of relevance is that two different densities of cotton were equally effective (100%) in preventing contamination regardless of density (120-vs. 200 g/m(2); T-test, p = 0.0028), suggesting that similar density materials could prevent droplet contamination. As a practical message, we conducted a speech trial (counting numbers, 1-100) with/without the protection of the same cotton textile used as face cover. The trial illustrated that contamination of surfaces occurs at a rate of >2-6 bacteria-carrying saliva-droplets per word (2.6 droplets/cm(2), 30 cm) when speaking at 60-70 decibels and that cotton face covers fully prevent bacterial surface contamination.
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