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10.1016/j.jviromet.2020.113977

http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.113977
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32979405!7510776!32979405
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suck abstract from ncbi


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pmid32979405      J+Virol+Methods 2020 ; 286 (ä): 113977
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  • Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation #MMPMID32979405
  • Alexander MR; Rootes CL; van Vuren PJ; Stewart CR
  • J Virol Methods 2020[Dec]; 286 (ä): 113977 PMID32979405show ga
  • The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus precipitation resulted in a approximately 1.5 log(10) increase in SARS-CoV-2 titres of virus prepared in VeroE6 cells and enabled the infection of several immortalized human cell lines (Caco-2 and Calu-3) at a high multiplicity of infection not practically achievable without virus concentration. This protocol underscores the utility of PEG-mediated precipitation for SARS-CoV-2 and provides a resource for a range of coronavirus research areas.
  • |Animals[MESH]
  • |Betacoronavirus/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Caco-2 Cells[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/diagnosis/*virology[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/diagnosis/*virology[MESH]
  • |Polyethylene Glycols/*chemistry[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]
  • |Ultracentrifugation/methods[MESH]


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