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10.1038/s41596-020-0394-5 http://scihub22266oqcxt.onion/10.1038/s41596-020-0394-5 32978602!ä!32978602 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2020 ; 15 (11): 3699-3715 Nephropedia Template TP {{tp|p=32978602|t=2020. Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped virus-based assay |pdf=|usr=}}{{32978602}} gab.com Text Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped virus-based assay JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=32978602 #FOAvip Pseudotyped viruses are useful virological tools because of their safety and versatility. On the basis of a vesicular stomatitis virus (VSV) pseudotyped virus production system, we developed a pseudotyped virus-based neutralization assay against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in biosafety level 2 facilities. Compared with the binding antibody test, the neutralization assay could discriminate the protective agents from the antibody family. This protocol includes production and titration of the SARS-CoV-2 S pseudotyped virus and the neutralization assay based on it. Various types of samples targeting virus attachment and entry could be evaluated for their potency, including serum samples derived from animals and humans, monoclonal antibodies and fusion inhibitors (peptides or small molecules). If the pseudotyped virus stock has been prepared in advance, it will take 2 days to get the potency data for the candidate samples. Experience in handling cells is needed before implementing this protocol. Twit Text FOAVip Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped virus-based assay ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=32978602 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32978602 #FOAvip English Wikipedia <ref>{{Cite pmid|32978602}}</ref> Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped virus-based assay #MMPMID32978602 Nie J; Li Q; Wu J; Zhao C; Hao H; Liu H; Zhang L; Nie L; Qin H; Wang M; Lu Q; Li X; Sun Q; Liu J; Fan C; Huang W; Xu M; Wang Y Nat Protoc 2020[Nov]; 15 (11): 3699-3715 PMID32978602show ga Pseudotyped viruses are useful virological tools because of their safety and versatility. On the basis of a vesicular stomatitis virus (VSV) pseudotyped virus production system, we developed a pseudotyped virus-based neutralization assay against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in biosafety level 2 facilities. Compared with the binding antibody test, the neutralization assay could discriminate the protective agents from the antibody family. This protocol includes production and titration of the SARS-CoV-2 S pseudotyped virus and the neutralization assay based on it. Various types of samples targeting virus attachment and entry could be evaluated for their potency, including serum samples derived from animals and humans, monoclonal antibodies and fusion inhibitors (peptides or small molecules). If the pseudotyped virus stock has been prepared in advance, it will take 2 days to get the potency data for the candidate samples. Experience in handling cells is needed before implementing this protocol. |*Genetic Techniques[MESH] |Animals[MESH] |Antibodies, Neutralizing/*analysis[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |Coronavirus Infections/*virology[MESH] |Female[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Mice[MESH] |Pandemics[MESH] |Pneumonia, Viral/*virology[MESH]Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped vi(epmc).pdf DeepDyve Pubget Overpricing