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10.3390/v12091054 http://scihub22266oqcxt.onion/10.3390/v12091054 32971895!7551300!32971895     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (9): ä Nephropedia Template TP {{tp|p=32971895|t=2020. Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus |pdf=|usr=}}{{32971895}} gab.com Text Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32971895 #FOAvip Coronaviruses (CoVs) are enveloped, positive sense, single strand RNA viruses that cause respiratory, intestinal and neurological diseases in mammals and birds. Following replication, CoVs assemble on intracellular membranes including the endoplasmic reticulum Golgi intermediate compartment (ERGIC) where the envelope protein (E) functions in virus assembly and release. In consequence, E potentially contains membrane-modifying peptides. To search for such peptides, the E coding sequence of Mouse Hepatitis Virus (MHV) was inspected for its amino acid conservation, proximity to the membrane and/or predicted amphipathic helices. Peptides identified in silico were synthesized and tested for membrane-modifying activity in the presence of giant unilamellar vesicles (GUVs) consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), sphingomyelin and cholesterol. To confirm the presence of membrane binding peptides identified in the context of a full-length E protein, the wild type and a number of mutants in the putative membrane binding peptide were expressed in Lenti-X-293T mammalian and insect cells, and the distribution of E antigen within the expressing cell was assessed. Our data identify a role for the post-transmembrane region of MHV E in membrane binding. Twit Text FOAVip Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32971895 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32971895 #FOAvip English Wikipedia <ref>{{Cite pmid|32971895}}</ref> Identification of a Membrane Binding Peptide in the Envelope Protein of MHV Coronavirus #MMPMID32971895 Alsaadi EAJ; Neuman BW; Jones IM Viruses 2020[Sep]; 12 (9): ä PMID32971895show ga Coronaviruses (CoVs) are enveloped, positive sense, single strand RNA viruses that cause respiratory, intestinal and neurological diseases in mammals and birds. Following replication, CoVs assemble on intracellular membranes including the endoplasmic reticulum Golgi intermediate compartment (ERGIC) where the envelope protein (E) functions in virus assembly and release. In consequence, E potentially contains membrane-modifying peptides. To search for such peptides, the E coding sequence of Mouse Hepatitis Virus (MHV) was inspected for its amino acid conservation, proximity to the membrane and/or predicted amphipathic helices. Peptides identified in silico were synthesized and tested for membrane-modifying activity in the presence of giant unilamellar vesicles (GUVs) consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), sphingomyelin and cholesterol. To confirm the presence of membrane binding peptides identified in the context of a full-length E protein, the wild type and a number of mutants in the putative membrane binding peptide were expressed in Lenti-X-293T mammalian and insect cells, and the distribution of E antigen within the expressing cell was assessed. Our data identify a role for the post-transmembrane region of MHV E in membrane binding. |Amino Acid Sequence[MESH] |Animals[MESH] |Cell Line[MESH] |Coronavirus Infections[MESH] |Humans[MESH] |Intracellular Membranes/metabolism[MESH] |Mice[MESH] |Murine hepatitis virus/*chemistry/genetics/metabolism[MESH] |Mutation[MESH] |Peptides/chemical synthesis/*chemistry/metabolism[MESH] |Sf9 Cells[MESH] |Spodoptera[MESH] |Unilamellar Liposomes/metabolism[MESH]Identification of a Membrane Binding Peptide in the Envelope Protein o(epmc).pdf DeepDyve Pubget Overpricing