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10.1080/22221751.2020.1826892

http://scihub22266oqcxt.onion/10.1080/22221751.2020.1826892
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suck abstract from ncbi


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pmid32969769      Emerg+Microbes+Infect 2020 ; 9 (1): 2212-2221
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  • Rapid Determination of SARS-CoV-2 antibodies using a bedside, point-of-Care, serological test #MMPMID32969769
  • Dortet L; Emeraud C; Vauloup-Fellous C; Khecharem M; Ronat JB; Fortineau N; Roque-Afonso AM; Naas T
  • Emerg Microbes Infect 2020[Dec]; 9 (1): 2212-2221 PMID32969769show ga
  • Background: Several serological tests for SARS-CoV-2 have been developed or use, but most have only been validated on few samples, and none provide medical practitioners with an easy-to-use, self-contained, bedside test with high accuracy. Material and methods: Two-hundred fifty-six sera from 101 patients hospitalized with SARS-CoV-2 infection (positive RT-PCR) and 50 control sera were tested for IgM/IgG using the NG-Test IgM-IgG COVID all-in-one assay. The seroconversion dynamic was assessed by symptom onset and day of RT-PCR diagnosis. Results: Among the SARS-CoV-2 infected patients, positive IgG and/or IgM result was observed for 67.3% of patients (68/101), including 17 (16.8%) already positive at the day of RT-PCR, and 51 (50.5%) with observable seroconversion, and 32.7% (33/101) remained negative as subsequent sampling was not possible (patient discharge or death). The sensitivity increased with the delay between onset of symptoms and sampling, going from 29.1%, 78.2% and 86.5% for the time periods of 0-9-, 10-14- and >14-days after the onset of symptoms, respectively. Cumulative sensitivity, specificity, Positive Predictive Value and Negative Predictive Value were 97.0%, 100%, 100% and 96.2%, respectively 15-days after the onset of symptoms. No difference in seroconversion delay was observed regardless of whether patients received ventilation. Conclusions: The NG-test is a bedside serological assay that could serve as a complementary source of diagnostic information to RT-PCR and chest imaging. It may also be useful to monitor immunological status of medical and non-medical workers during the ongoing pandemic, and the general population after social distancing measures have eased.
  • |*Clinical Laboratory Techniques[MESH]
  • |*Point-of-Care Testing[MESH]
  • |Adult[MESH]
  • |Antibodies, Viral/blood/*immunology[MESH]
  • |Betacoronavirus/genetics/*immunology[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Case-Control Studies[MESH]
  • |Coronavirus Infections/*diagnosis/*immunology/virology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoglobulin G/blood/immunology[MESH]
  • |Immunoglobulin M/blood/immunology[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/*immunology/virology[MESH]
  • |Polymerase Chain Reaction[MESH]
  • |Reagent Strips[MESH]
  • |SARS-CoV-2[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Seroconversion[MESH]


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