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10.1038/s41467-020-18611-5 http://scihub22266oqcxt.onion/10.1038/s41467-020-18611-5 32968075!7511968!32968075     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2020 ; 11 (1): 4812 Nephropedia Template TP {{tp|p=32968075|t=2020. Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR |pdf=|usr=}}{{32968075}} gab.com Text Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=32968075 #FOAvip Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction constitutes a major bottleneck in current testing. Methodological simplification could increase diagnostic availability and efficiency, benefitting patient care and infection control. Here, we describe methods circumventing RNA extraction in COVID-19 testing by performing RT-PCR directly on heat-inactivated or lysed samples. Our data, including benchmarking using 597 clinical patient samples and a standardised diagnostic system, demonstrate that direct RT-PCR is viable option to extraction-based tests. Using controlled amounts of active SARS-CoV-2, we confirm effectiveness of heat inactivation by plaque assay and evaluate various generic buffers as transport medium for direct RT-PCR. Significant savings in time and cost are achieved through RNA-extraction-free protocols that are directly compatible with established PCR-based testing pipelines. This could aid expansion of COVID-19 testing. Twit Text FOAVip Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=32968075 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32968075 #FOAvip English Wikipedia <ref>{{Cite pmid|32968075}}</ref> Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR #MMPMID32968075 Smyrlaki I; Ekman M; Lentini A; Rufino de Sousa N; Papanicolaou N; Vondracek M; Aarum J; Safari H; Muradrasoli S; Rothfuchs AG; Albert J; Hogberg B; Reinius B Nat Commun 2020[Sep]; 11 (1): 4812 PMID32968075show ga Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction constitutes a major bottleneck in current testing. Methodological simplification could increase diagnostic availability and efficiency, benefitting patient care and infection control. Here, we describe methods circumventing RNA extraction in COVID-19 testing by performing RT-PCR directly on heat-inactivated or lysed samples. Our data, including benchmarking using 597 clinical patient samples and a standardised diagnostic system, demonstrate that direct RT-PCR is viable option to extraction-based tests. Using controlled amounts of active SARS-CoV-2, we confirm effectiveness of heat inactivation by plaque assay and evaluate various generic buffers as transport medium for direct RT-PCR. Significant savings in time and cost are achieved through RNA-extraction-free protocols that are directly compatible with established PCR-based testing pipelines. This could aid expansion of COVID-19 testing. |Benchmarking[MESH] |Betacoronavirus/*genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods/standards/statistics & numerical data[MESH] |Coronavirus Infections/*diagnosis/epidemiology/*virology[MESH] |DNA Primers/genetics[MESH] |Hot Temperature[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/*virology[MESH] |RNA, Viral/genetics/isolation & purification[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods/standards/statistics & numerical data[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Sweden/epidemiology[MESH]Massive and rapid COVID-19 testing is feasible by extraction-free SARS(epmc).pdf DeepDyve Pubget Overpricing