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10.1002/jmv.26540 http://scihub22266oqcxt.onion/10.1002/jmv.26540 32966645!7537076!32966645     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (3): 1694-1701 Nephropedia Template TP {{tp|p=32966645|t=2021. One-step quantitative RT-PCR assay with armored RNA controls for detection of SARS-CoV-2 |pdf=|usr=}}{{32966645}} gab.com Text One-step quantitative RT-PCR assay with armored RNA controls for detection of SARS-CoV-2 JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32966645 #FOAvip Coronavirus disease 2019 (COVID-19) has become pandemic since March 11, 2020. Thus, development and integration in clinics of fast and sensitive diagnostic tools are essential. The aim of the study is a development and evaluation of a one-step quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay (COVID-19 Amp) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection with an armored positive control and internal controls constructed from synthetic MS2-phage-based RNA particles. The COVID-19 Amp assay limit of detection was 10(3) copies/ml, the analytical specificity was 100%. A total of 109 biological samples were examined using COVID-19 Amp and World Health Organization (WHO)-based assay. Discordance in nine samples was observed (negative by the WHO-based assay) and discordant samples were retested as positive according to the results obtained from the Vector-PCRrv-2019-nCoV-RG assay. The developed COVID-19 Amp assay has high sensitivity and specificity, includes virus particles-based controls, provides the direct definition of the SARS-CoV-2 RdRp gene partial sequence, and is suitable for any hospital and laboratory equipped for RT-qPCR. Twit Text FOAVip One-step quantitative RT-PCR assay with armored RNA controls for detection of SARS-CoV-2 ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32966645 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32966645 #FOAvip English Wikipedia <ref>{{Cite pmid|32966645}}</ref> One-step quantitative RT-PCR assay with armored RNA controls for detection of SARS-CoV-2 #MMPMID32966645 Goncharova EA; Dedkov VG; Dolgova AS; Kassirov IS; Safonova MV; Voytsekhovskaya Y; Totolian AA J Med Virol 2021[Mar]; 93 (3): 1694-1701 PMID32966645show ga Coronavirus disease 2019 (COVID-19) has become pandemic since March 11, 2020. Thus, development and integration in clinics of fast and sensitive diagnostic tools are essential. The aim of the study is a development and evaluation of a one-step quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay (COVID-19 Amp) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection with an armored positive control and internal controls constructed from synthetic MS2-phage-based RNA particles. The COVID-19 Amp assay limit of detection was 10(3) copies/ml, the analytical specificity was 100%. A total of 109 biological samples were examined using COVID-19 Amp and World Health Organization (WHO)-based assay. Discordance in nine samples was observed (negative by the WHO-based assay) and discordant samples were retested as positive according to the results obtained from the Vector-PCRrv-2019-nCoV-RG assay. The developed COVID-19 Amp assay has high sensitivity and specificity, includes virus particles-based controls, provides the direct definition of the SARS-CoV-2 RdRp gene partial sequence, and is suitable for any hospital and laboratory equipped for RT-qPCR. |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Diagnostic Tests, Routine[MESH] |Female[MESH] |Genome, Viral/genetics[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |RNA, Viral/genetics[MESH] |RNA-Dependent RNA Polymerase/genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Sensitivity and Specificity[MESH]One-step quantitative RT-PCR assay with armored RNA controls for detec(epmc).pdf DeepDyve Pubget Overpricing