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10.1007/s00424-020-02457-3 http://scihub22266oqcxt.onion/10.1007/s00424-020-02457-3 32964285!7561021!32964285     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Pflugers+Arch 2020 ; 472 (11): 1589-1606 Nephropedia Template TP {{tp|p=32964285|t=2020. TRPM7 channel activity in Jurkat T lymphocytes during magnesium depletion and loading: implications for divalent metal entry and cytotoxicity |pdf=|usr=}}{{32964285}} gab.com Text TRPM7 channel activity in Jurkat T lymphocytes during magnesium depletion and loading: implications for divalent metal entry and cytotoxicity JO: Pflugers Arch http://www.kidney.de/pget.php?&tw=1&pmid=32964285 #FOAvip TRPM7 is a cation channel-protein kinase highly expressed in T lymphocytes and other immune cells. It has been proposed to constitute a cellular entry pathway for Mg(2+) and divalent metal cations such as Ca(2+), Zn(2+), Cd(2+), Mn(2+), and Ni(2+). TRPM7 channels are inhibited by cytosolic Mg(2+), rendering them largely inactive in intact cells. The dependence of channel activity on extracellular Mg(2+) is less well studied. Here, we measured native TRPM7 channel activity in Jurkat T cells maintained in external Mg(2+) concentrations varying between 400 nM and 1.4 mM for 1-3 days, obtaining an IC(50) value of 54 muM. Maintaining the cells in 400 nM or 8 muM [Mg(2+)](o) resulted in almost complete activation of TRPM7 in intact cells, due to cytosolic Mg(2+) depletion. A total of 1.4 mM [Mg(2+)](o) was sufficient to fully eliminate the basal current. Submillimolar concentrations of amiloride prevented cellular Mg(2+) depletion but not loading. We investigated whether the cytotoxicity of TRPM7 permeant metal ions Ni(2+), Zn(2+), Cd(2+), Co(2+), Mn(2+), Sr(2+), and Ba(2+) requires TRPM7 channel activity. Mg(2+) loading modestly reduced cytotoxicity of Zn(2+), Co(2+), Ni(2+), and Mn(2+) but not of Cd(2+). Channel blocker NS8593 reduced Co(2+) and Mn(2+) but not Cd(2+) or Zn(2+) cytotoxicity and interfered with Mg(2+) loading as evaluated by TRPM7 channel basal activity. Ba(2+) and Sr(2+) were neither detectably toxic nor permeant through the plasma membrane. These results indicate that in Jurkat T cells, entry of toxic divalent metal cations primarily occurs through pathways distinct from TRPM7. By contrast, we found evidence that Mg(2+) entry requires TRPM7 channels. Twit Text FOAVip TRPM7 channel activity in Jurkat T lymphocytes during magnesium depletion and loading: implications for divalent metal entry and cytotoxicity ????Pflugers Arch http://www.kidney.de/pget.php?&tw=1&pmid=32964285 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32964285 #FOAvip English Wikipedia <ref>{{Cite pmid|32964285}}</ref> TRPM7 channel activity in Jurkat T lymphocytes during magnesium depletion and loading: implications for divalent metal entry and cytotoxicity #MMPMID32964285 Mellott A; Rockwood J; Zhelay T; Luu CT; Kaitsuka T; Kozak JA Pflugers Arch 2020[Nov]; 472 (11): 1589-1606 PMID32964285show ga TRPM7 is a cation channel-protein kinase highly expressed in T lymphocytes and other immune cells. It has been proposed to constitute a cellular entry pathway for Mg(2+) and divalent metal cations such as Ca(2+), Zn(2+), Cd(2+), Mn(2+), and Ni(2+). TRPM7 channels are inhibited by cytosolic Mg(2+), rendering them largely inactive in intact cells. The dependence of channel activity on extracellular Mg(2+) is less well studied. Here, we measured native TRPM7 channel activity in Jurkat T cells maintained in external Mg(2+) concentrations varying between 400 nM and 1.4 mM for 1-3 days, obtaining an IC(50) value of 54 muM. Maintaining the cells in 400 nM or 8 muM [Mg(2+)](o) resulted in almost complete activation of TRPM7 in intact cells, due to cytosolic Mg(2+) depletion. A total of 1.4 mM [Mg(2+)](o) was sufficient to fully eliminate the basal current. Submillimolar concentrations of amiloride prevented cellular Mg(2+) depletion but not loading. We investigated whether the cytotoxicity of TRPM7 permeant metal ions Ni(2+), Zn(2+), Cd(2+), Co(2+), Mn(2+), Sr(2+), and Ba(2+) requires TRPM7 channel activity. Mg(2+) loading modestly reduced cytotoxicity of Zn(2+), Co(2+), Ni(2+), and Mn(2+) but not of Cd(2+). Channel blocker NS8593 reduced Co(2+) and Mn(2+) but not Cd(2+) or Zn(2+) cytotoxicity and interfered with Mg(2+) loading as evaluated by TRPM7 channel basal activity. Ba(2+) and Sr(2+) were neither detectably toxic nor permeant through the plasma membrane. These results indicate that in Jurkat T cells, entry of toxic divalent metal cations primarily occurs through pathways distinct from TRPM7. By contrast, we found evidence that Mg(2+) entry requires TRPM7 channels. |1-Naphthylamine/analogs & derivatives/pharmacology[MESH] |Action Potentials[MESH] |Humans[MESH] |Inhibitory Concentration 50[MESH] |Ion Transport[MESH] |Jurkat Cells[MESH] |Magnesium/*metabolism[MESH] |Metals, Heavy/metabolism/*toxicity[MESH] |Protein Serine-Threonine Kinases/antagonists & inhibitors/*metabolism[MESH]TRPM7 channel activity in Jurkat T lymphocytes during magnesium deplet(epmc).pdf DeepDyve Pubget Overpricing