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10.1080/22221751.2020.1826362

http://scihub22266oqcxt.onion/10.1080/22221751.2020.1826362
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suck abstract from ncbi


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pmid32962560      Emerg+Microbes+Infect 2020 ; 9 (1): 2200-2211
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  • Meta-analysis of diagnostic performance of serology tests for COVID-19: impact of assay design and post-symptom-onset intervals #MMPMID32962560
  • Wang H; Ai J; Loeffelholz MJ; Tang YW; Zhang W
  • Emerg Microbes Infect 2020[Dec]; 9 (1): 2200-2211 PMID32962560show ga
  • Serology detection is recognized for its sensitivity in convalescent patients with COVID-19, in comparison with nucleic acid amplification tests (NAATs). This article aimed to evaluate the diagnostic accuracy of serologic methods for COVID-19 based on assay design and post-symptom-onset intervals. Two authors independently searched PubMed, Cochrane library, Ovid, EBSCO for case-control, longitudinal and cohort studies that determined the diagnostic accuracy of serology tests in comparison with NAATs in COVID-19 cases and used QUADAS-2 for quality assessment. Pooled accuracy was analysed using INLA method. A total of 27 studies were included in this meta-analysis, with 4 cohort, 16 case-control and 7 longitudinal studies and 4565 participants. Serology tests had the lowest sensitivity at 0-7 days after symptom onset and the highest at >14 days. TAB had a better sensitivity than IgG or IgM only. Using combined nucleocapsid (N) and spike(S) protein had a better sensitivity compared to N or S protein only. Lateral flow immunoassay (LFIA) had a lower sensitivity than enzyme-linked immunoassay (ELISA) and chemiluminescent immunoassay (CLIA). Serology tests will play an important role in the clinical diagnosis for later stage COVID-19 patients. ELISA tests, detecting TAB or targeting combined N and S proteins had a higher diagnostic sensitivity compared to other methods.
  • |*Betacoronavirus/immunology[MESH]
  • |Antibodies, Viral/blood/immunology[MESH]
  • |Antigens, Viral/immunology[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods/standards[MESH]
  • |Coronavirus Infections/*diagnosis/immunology/*virology[MESH]
  • |Humans[MESH]
  • |Immunoassay/methods/standards[MESH]
  • |Immunoglobulin G/blood/immunology[MESH]
  • |Immunoglobulin M/blood/immunology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/immunology/*virology[MESH]
  • |Publication Bias[MESH]
  • |SARS-CoV-2[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Serologic Tests/*methods/standards[MESH]


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  • suck abstract from ncbi

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