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10.1016/j.diagmicrobio.2020.115181

http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2020.115181
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32957073!7441068!32957073
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suck abstract from ncbi


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pmid32957073      Diagn+Microbiol+Infect+Dis 2020 ; 98 (4): 115181
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  • Evaluation of the performance of SARS-CoV-2 serological tools and their positioning in COVID-19 diagnostic strategies #MMPMID32957073
  • Velay A; Gallais F; Benotmane I; Wendling MJ; Danion F; Collange O; De Seze J; Schmidt-Mutter C; Schneider F; Bilbault P; Meziani F; Fafi-Kremer S
  • Diagn Microbiol Infect Dis 2020[Dec]; 98 (4): 115181 PMID32957073show ga
  • Rapid and accurate diagnosis is crucial for successful outbreak containment. During the current coronavirus disease 2019 (COVID-19) public health emergency, the gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection diagnosis is the detection of viral RNA. Additional diagnostic methods oenabling the detection of current or past SARS-CoV-2 infection would be highly beneficial. We assessed 2 immunochromatographic lateral flow assays (LFA-1, LFA-2) and 2 enzyme-linked immunosorbent assay kits (IgA/IgG ELISA-1, IgM/IgG ELISA-2) using 325 samples: serum samples from polymerase chain reaction-confirmed COVID-19 hospitalized patients (n?=?55) and healthcare workers (n?=?143) and 127 samples from negative controls. Diagnostic performances were assessed according to days after symptom onset (dso) and the antigenic format used by manufacturers. Clinical sensitivities varied greatly among the assays, showing poor mutual agreement. After 15 dso, ELISA-1 (Euroimmun) and LFA-1 (Biosynex) combining IgM and IgG detection showed the best performances. A thorough selection of serological assays for the detection of ongoing or past infections is advisable.
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |Betacoronavirus/immunology[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |Enzyme-Linked Immunosorbent Assay/*methods[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoassay/*methods[MESH]
  • |Immunoglobulin A/blood[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Immunoglobulin M/blood[MESH]
  • |Male[MESH]
  • |Mass Screening/*methods[MESH]
  • |Middle Aged[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |Polymerase Chain Reaction[MESH]
  • |RNA, Viral/analysis[MESH]
  • |SARS-CoV-2[MESH]
  • |Sensitivity and Specificity[MESH]


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