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10.1016/j.xpro.2020.100102

http://scihub22266oqcxt.onion/10.1016/j.xpro.2020.100102
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32954369!7490627!32954369
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suck abstract from ncbi


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pmid32954369      STAR+Protoc 2020 ; 1 (2): 100102
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  • qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples #MMPMID32954369
  • Giadone RM; Mithal A; Miller GJ; Matte TM; Yeung AK; Dowrey TW; Werder RB; Miller NS; Andry CD; Vanuytsel K; Murphy GJ
  • STAR Protoc 2020[Sep]; 1 (2): 100102 PMID32954369show ga
  • The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time. For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020).
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Disease Notification/*methods[MESH]
  • |Humans[MESH]
  • |Real-Time Polymerase Chain Reaction/*methods[MESH]
  • |SARS-CoV-2/*genetics/isolation & purification[MESH]


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