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10.1007/s00216-020-02930-z http://scihub22266oqcxt.onion/10.1007/s00216-020-02930-z 32951064!7502157!32951064     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32951064&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Anal+Bioanal+Chem 2020 ; 412 (28): 7977-7988 Nephropedia Template TP {{tp|p=32951064|t=2020. Affine analysis for quantitative PCR measurements |pdf=|usr=}}{{32951064}} gab.com Text Affine analysis for quantitative PCR measurements JO: Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=32951064 #FOAvip Motivated by the current COVID-19 health crisis, we consider data analysis for quantitative polymerase chain-reaction (qPCR) measurements. We derive a theoretical result specifying the conditions under which all qPCR amplification curves (including their plateau phases) are identical up to an affine transformation, i.e. a multiplicative factor and horizontal shift. We use this result to develop a data analysis procedure for determining when an amplification curve exhibits characteristics of a true signal. The main idea behind this approach is to invoke a criterion based on constrained optimization that assesses when a measurement signal can be mapped to a master reference curve. We demonstrate that this approach: (i) can decrease the fluorescence detection threshold by up to a decade; and (ii) simultaneously improve confidence in interpretations of late-cycle amplification curves. Moreover, we demonstrate that the master curve is transferable reference data that can harmonize analyses between different labs and across several years. Application to reverse-transcriptase qPCR measurements of a SARS-CoV-2 RNA construct points to the usefulness of this approach for improving confidence and reducing limits of detection in diagnostic testing of emerging diseases. Graphical Abstract Left: a collection of qPCR amplification curves. Right: Example of data collapse after affine transformation. Twit Text FOAVip Affine analysis for quantitative PCR measurements ????Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=32951064 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32951064 #FOAvip English Wikipedia <ref>{{Cite pmid|32951064}}</ref> Affine analysis for quantitative PCR measurements #MMPMID32951064 Patrone PN; Romsos EL; Cleveland MH; Vallone PM; Kearsley AJ Anal Bioanal Chem 2020[Nov]; 412 (28): 7977-7988 PMID32951064show ga Motivated by the current COVID-19 health crisis, we consider data analysis for quantitative polymerase chain-reaction (qPCR) measurements. We derive a theoretical result specifying the conditions under which all qPCR amplification curves (including their plateau phases) are identical up to an affine transformation, i.e. a multiplicative factor and horizontal shift. We use this result to develop a data analysis procedure for determining when an amplification curve exhibits characteristics of a true signal. The main idea behind this approach is to invoke a criterion based on constrained optimization that assesses when a measurement signal can be mapped to a master reference curve. We demonstrate that this approach: (i) can decrease the fluorescence detection threshold by up to a decade; and (ii) simultaneously improve confidence in interpretations of late-cycle amplification curves. Moreover, we demonstrate that the master curve is transferable reference data that can harmonize analyses between different labs and across several years. Application to reverse-transcriptase qPCR measurements of a SARS-CoV-2 RNA construct points to the usefulness of this approach for improving confidence and reducing limits of detection in diagnostic testing of emerging diseases. Graphical Abstract Left: a collection of qPCR amplification curves. Right: Example of data collapse after affine transformation. |*Algorithms[MESH] |Betacoronavirus/*genetics/isolation & purification[MESH] |COVID-19[MESH] |Coronavirus Infections/diagnosis/*virology[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/diagnosis/*virology[MESH] |RNA, Viral/analysis/*genetics[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]Affine analysis for quantitative PCR measurements (epmc).pdf DeepDyve Pubget Overpricing