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10.1016/j.meegid.2020.104557 http://scihub22266oqcxt.onion/10.1016/j.meegid.2020.104557 32950697!7497549!32950697     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Infect+Genet+Evol 2020 ; 85 (ä): 104557 Nephropedia Template TP {{tp|p=32950697|t=2020. Substitutions in Spike and Nucleocapsid proteins of SARS-CoV-2 circulating in South America |pdf=|usr=}}{{32950697}} gab.com Text Substitutions in Spike and Nucleocapsid proteins of SARS-CoV-2 circulating in South America JO: Infect Genet Evol http://www.kidney.de/pget.php?&tw=1&pmid=32950697 #FOAvip SARS-CoV-2 is a new member of the genus Betacoronavirus, responsible for the COVID-19 pandemic. The virus crossed the species barrier and established in the human population taking advantage of the spike protein high affinity for the ACE receptor to infect the lower respiratory tract. The Nucleocapsid (N) and Spike (S) are highly immunogenic structural proteins and most commercial COVID-19 diagnostic assays target these proteins. In an unpredictable epidemic, it is essential to know about their genetic variability. The objective of this study was to describe the substitution frequency of the S and N proteins of SARS-CoV-2 in South America. A total of 504 amino acid and nucleotide sequences of the S and N proteins of SARS-CoV-2 from seven South American countries (Argentina, Brazil, Chile, Ecuador, Peru, Uruguay, and Colombia), reported as of June 3, and corresponding to samples collected between March and April 2020, were compared through substitution matrices using the Muscle algorithm. Forty-three sequences from 13 Colombian departments were obtained in this study using the Oxford Nanopore and Illumina MiSeq technologies, following the amplicon-based ARTIC network protocol. The substitutions D614G in S and R203K/G204R in N were the most frequent in South America, observed in 83% and 34% of the sequences respectively. Strikingly, genomes with the conserved position D614 were almost completely replaced by genomes with the G614 substitution between March to April 2020. A similar replacement pattern was observed with R203K/G204R although more marked in Chile, Argentina and Brazil, suggesting similar introduction history and/or control strategies of SARS-CoV-2 in these countries. It is necessary to continue with the genomic surveillance of S and N proteins during the SARS-CoV-2 pandemic as this information can be useful for developing vaccines, therapeutics and diagnostic tests. Twit Text FOAVip Substitutions in Spike and Nucleocapsid proteins of SARS-CoV-2 circulating in South America ????Infect Genet Evol http://www.kidney.de/pget.php?&tw=1&pmid=32950697 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32950697 #FOAvip English Wikipedia <ref>{{Cite pmid|32950697}}</ref> Substitutions in Spike and Nucleocapsid proteins of SARS-CoV-2 circulating in South America #MMPMID32950697 Franco-Munoz C; Alvarez-Diaz DA; Laiton-Donato K; Wiesner M; Escandon P; Usme-Ciro JA; Franco-Sierra ND; Florez-Sanchez AC; Gomez-Rangel S; Rodriguez-Calderon LD; Barbosa-Ramirez J; Ospitia-Baez E; Walteros DM; Ospina-Martinez ML; Mercado-Reyes M Infect Genet Evol 2020[Nov]; 85 (ä): 104557 PMID32950697show ga SARS-CoV-2 is a new member of the genus Betacoronavirus, responsible for the COVID-19 pandemic. The virus crossed the species barrier and established in the human population taking advantage of the spike protein high affinity for the ACE receptor to infect the lower respiratory tract. The Nucleocapsid (N) and Spike (S) are highly immunogenic structural proteins and most commercial COVID-19 diagnostic assays target these proteins. In an unpredictable epidemic, it is essential to know about their genetic variability. The objective of this study was to describe the substitution frequency of the S and N proteins of SARS-CoV-2 in South America. A total of 504 amino acid and nucleotide sequences of the S and N proteins of SARS-CoV-2 from seven South American countries (Argentina, Brazil, Chile, Ecuador, Peru, Uruguay, and Colombia), reported as of June 3, and corresponding to samples collected between March and April 2020, were compared through substitution matrices using the Muscle algorithm. Forty-three sequences from 13 Colombian departments were obtained in this study using the Oxford Nanopore and Illumina MiSeq technologies, following the amplicon-based ARTIC network protocol. The substitutions D614G in S and R203K/G204R in N were the most frequent in South America, observed in 83% and 34% of the sequences respectively. Strikingly, genomes with the conserved position D614 were almost completely replaced by genomes with the G614 substitution between March to April 2020. A similar replacement pattern was observed with R203K/G204R although more marked in Chile, Argentina and Brazil, suggesting similar introduction history and/or control strategies of SARS-CoV-2 in these countries. It is necessary to continue with the genomic surveillance of S and N proteins during the SARS-CoV-2 pandemic as this information can be useful for developing vaccines, therapeutics and diagnostic tests. |*Amino Acid Substitution[MESH] |COVID-19/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins/genetics[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |Phylogeny[MESH] |SARS-CoV-2/*classification/genetics[MESH] |Sequence Analysis, RNA[MESH] |South America[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH]Substitutions in Spike and Nucleocapsid proteins of SARS-CoV-2 circula(epmc).pdf DeepDyve Pubget Overpricing