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10.1016/j.ijid.2020.09.025 http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.09.025 32949774!7493737!32949774     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32949774&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Infect+Dis 2020 ; 100 (ä): 476-482 Nephropedia Template TP {{tp|p=32949774|t=2020. Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients |pdf=|usr=}}{{32949774}} gab.com Text Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32949774 #FOAvip OBJECTIVES: Because the detection of SARS-CoV-2 RNA in aerosols but failure to isolate viable (infectious) virus are commonly reported, there is substantial controversy whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be transmitted through aerosols. This conundrum occurs because common air samplers can inactivate virions through their harsh collection processes. We sought to resolve the question whether viable SARS-CoV-2 can occur in aerosols using VIVAS air samplers that operate on a gentle water vapor condensation principle. METHODS: Air samples collected in the hospital room of two coronavirus disease-2019 (COVID-19) patients, one ready for discharge and the other newly admitted, were subjected to RT-qPCR and virus culture. The genomes of the SARS-CoV-2 collected from the air and isolated in cell culture were sequenced. RESULTS: Viable SARS-CoV-2 was isolated from air samples collected 2 to 4.8 m away from the patients. The genome sequence of the SARS-CoV-2 strain isolated from the material collected by the air samplers was identical to that isolated from the newly admitted patient. Estimates of viable viral concentrations ranged from 6 to 74 TCID(50) units/L of air. CONCLUSIONS: Patients with respiratory manifestations of COVID-19 produce aerosols in the absence of aerosol-generating procedures that contain viable SARS-CoV-2, and these aerosols may serve as a source of transmission of the virus. Twit Text FOAVip Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32949774 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32949774 #FOAvip English Wikipedia <ref>{{Cite pmid|32949774}}</ref> Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients #MMPMID32949774 Lednicky JA; Lauzard M; Fan ZH; Jutla A; Tilly TB; Gangwar M; Usmani M; Shankar SN; Mohamed K; Eiguren-Fernandez A; Stephenson CJ; Alam MM; Elbadry MA; Loeb JC; Subramaniam K; Waltzek TB; Cherabuddi K; Morris JG Jr; Wu CY Int J Infect Dis 2020[Nov]; 100 (ä): 476-482 PMID32949774show ga OBJECTIVES: Because the detection of SARS-CoV-2 RNA in aerosols but failure to isolate viable (infectious) virus are commonly reported, there is substantial controversy whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be transmitted through aerosols. This conundrum occurs because common air samplers can inactivate virions through their harsh collection processes. We sought to resolve the question whether viable SARS-CoV-2 can occur in aerosols using VIVAS air samplers that operate on a gentle water vapor condensation principle. METHODS: Air samples collected in the hospital room of two coronavirus disease-2019 (COVID-19) patients, one ready for discharge and the other newly admitted, were subjected to RT-qPCR and virus culture. The genomes of the SARS-CoV-2 collected from the air and isolated in cell culture were sequenced. RESULTS: Viable SARS-CoV-2 was isolated from air samples collected 2 to 4.8 m away from the patients. The genome sequence of the SARS-CoV-2 strain isolated from the material collected by the air samplers was identical to that isolated from the newly admitted patient. Estimates of viable viral concentrations ranged from 6 to 74 TCID(50) units/L of air. CONCLUSIONS: Patients with respiratory manifestations of COVID-19 produce aerosols in the absence of aerosol-generating procedures that contain viable SARS-CoV-2, and these aerosols may serve as a source of transmission of the virus. |*Air Microbiology[MESH] |Aerosols[MESH] |Betacoronavirus/*isolation & purification[MESH] |COVID-19[MESH] |Coronavirus Infections/transmission/*virology[MESH] |Hospitals[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/transmission/*virology[MESH]Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients(epmc).pdf DeepDyve Pubget Overpricing